File
Authors
Matsuura, Kazunori Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University Tottori University Researchers KAKEN Search Researchers
Mizuguchi, Yusaku Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University
Kimizuka, Nobuo Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University
Keywords
viral capsid
self-assembly
β-annulus peptide
nanosphere
NDC
Natural science
Abstract
A novel β-annulus peptide of Sesbania mosaic virus bearing an FKFE sequence at the C terminus was synthesized, and its self-assembling behavior in water was investigated. Dynamic light scattering and transmission electron microscopy showed that the β-annulus peptide bearing an FKFE sequence self-assembled into approximately 30 nm nanospheres in water at pH 3.8, whereas the β-annulus peptide without the FKFE sequence afforded only irregular aggregates. The peptide nanospheres possessed a definite critical aggregation concentration (CAC = 26 μM), above which the size of nanospheres were nearly unaffected by the peptide concentration. The formation of peptide nanospheres was significantly affected by pH; the peptide did not form any assemblies at pH 2.2 whereas larger aggregates were formed at pH 6.4–11.6.
Publisher
Wiley
Content Type
Journal Article
Link
ISSN・ISBN
00063525
NCID
AA11029495
Journal Title
Biopolymers. Peptide science
Current Journal Title
Biopolymers. Peptide science
Volume
106
Issue
4
Start Page
470
End Page
475
Published Date
2015-11-17
Publisher-DOI
Text Version
Author
Rights
© 2015 Wiley Periodicals, Inc.
Citation
This is the pre-peer reviewed version of the following article: Matsuura, K. , Mizuguchi, Y. and Kimizuka, N. (2016), Peptide nanospheres self‐assembled from a modified β‐annulus peptide of Sesbania mosaic virus. Biopolymers, 106: 470-475. which has been published in final form at https://doi.org/10.1002/bip.22774. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Department
Faculty of Engineering/Graduate School of Engineering
Language
English