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Authors
Izawa, Hironori Graduate School of Engineering, Tottori University
Okuda, Noriko Graduate School of Engineering, Tottori University
Yonemura, Tomoe Graduate School of Engineering, Tottori University
Kuroda, Kohei Department of Veterinary Clinical Medicine, Tottori University
Ochi, Kosuke Department of Veterinary Clinical Medicine, Tottori University
Ifuku, Shinsuke Graduate School of Engineering, Tottori University Tottori University Researchers KAKEN Search Researchers
Morimoto, Minoru Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University Tottori University Researchers KAKEN Search Researchers
Saimoto, Hiroyuki Graduate School of Engineering, Tottori University Tottori University Researchers KAKEN Search Researchers
Noda, Mayuko Department of Veterinary Clinical Medicine, Tottori University
Azuma, Kazuo Department of Veterinary Clinical Medicine, Tottori University Tottori University Researchers KAKEN Search Researchers
Okamoto, Yoshiharu Department of Veterinary Clinical Medicine, Tottori University Tottori University Researchers KAKEN Search Researchers
Ito, Norihiko Department of Veterinary Clinical Medicine, Tottori University Tottori University Researchers KAKEN Search Researchers
Keywords
chitosan
surface wrinkling
skin layer
horseradish peroxidase
cell culture substrate
3T3 cell
Abstract
Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 °C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress.
Publisher
MDPI AG
Content Type
Journal Article
Link
ISSN・ISBN
25045377
Journal Title
Colloids Interfaces
Volume
2
Issue
2
Start Page
15
Published Date
2018-03-26
Publisher-DOI
Text Version
Publisher
Rights
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Citation
IZAWA, Hironori, et al. Application of bio-based wrinkled surfaces as cell culture scaffolds. Colloids and Interfaces, 2018, 2.2: 15.
Department
Faculty of Engineering/Graduate School of Engineering
Language
English