File
Authors
Keywords
ATP-induced calcium mobilization
extracellular acidification
intracellular pH
rabbit lens epithelial cells
Abstract
Effects of extracellular acidification on intracellular pH (pHi) and ATP-induced calcium mobilization were investigated in rabbit lens epithelial cells. Primary-cultured lens epithelial cells of Japanese white rabbits were used. Intracellular calcium ([Ca2+]i) and pHi were measured by using fluorescent dyes, fura-2 acetoxymethylester (fura-2 AM) and 2',7'-bis (carboxyethyl)-5,6-carboxyfluorescein acetoxymethylester (BCECF AM), respectively. The addition of 10 ?mol/L ATP produced an initial peak followed by a sustained increase in [Ca2+]i in a standard artificial aqueous humor at extracellular pH (pH0) 7.40. The initial peak was abolished by pretreatment with 1 ?mol/L thapsigargin, whereas the sustained increase was attenuated in a Ca2+-free solution or by pretreatment with 100 ?mol/L verapamil. Acidification of the pH0 from 7.40 to 6.80 decreased the pHi from 7.21 to 7.03, and enhanced both the initial peak and sustained increase in [Ca2+]i. These results suggest that acidification of pH0 significantly affects the pHi and modifies the ATP-induced [Ca2+]i transient in rabbit lens epithelial cells.
Publisher
Tottori University Faculty of Medicine
Content Type
Journal Article
ISSN・ISBN
1346-8049
NCID
AA00892882
Journal Title
Yonago Acta medica
Current Journal Title
Yonago Acta medica
Volume
42
Issue
1
Start Page
51
End Page
59
Published Date
1999-03
Text Version
Publisher
Rights
Yonago Acta medica 編集委員会
Citation
Yonago Acta medica. 1999, 42(1), 51-59
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English