File
Authors
Keywords
Spirometra erinaceieuropaei
lipopolysaccharide
macrophage
cyclooxygenase-2
mitogen-activated protein kinase
Abstract
The escape mechanism of parasites from inflammatory processes has been thought to be one of the most important tools for their survival in infected tissues. On the other hand, inducible cyclooxygenase-2 (cox-2) has been shown to play a major role in the process of inflammation. We investigated the effect of excretory/secretory (ES) products from the plerocercoids of Spirometra erinaceieuropaei on cox-2 gene expression in a murine macrophage cell line (RAW 264.7). Preincubation of the macrophages with the ES products inhibited LPS-induced cox-2 mRNA expression by 75% as well as its protein production. Dibutyryl cAMP enhances LPS-induced cox-2 mRNA expression. The ES products also inhibited this enhanced expression by 46%. Inhibition of p38 mitogen-activated protein kinase (MAPK) with SB203580 or that of extracellular signal-regulated protein kinase with PD98059 reduced LPS-induced cox-2 mRNA expression by 75% or 52%, respectively, along with its protein production. This evidence suggests that both MAPK pathways are crucial for full induction of cox-2 gene expression. One experiment using a transcriptional inhibitor, actinomycin-D, showed that the ES products destabilized LPS-induced cox-2 mRNA in RAW 264.7 macrophages. These results show that ES products from the plerocercoids of Spirometra erinaceieuropaei suppress LPS-induced cox-2 mRNA expression in murine RAW 264.7 macrophages, and may attenuate inflammation around the plerocercoids of S. erinaceieuropaei.
Publisher
Tottori University Faculty of Medicine
Content Type
Journal Article
ISSN・ISBN
1346-8049
NCID
AA00892882
Journal Title
Yonago Acta medica
Current Journal Title
Yonago Acta medica
Volume
49
Issue
1
Start Page
39
End Page
47
Published Date
2006-03
Text Version
Publisher
Rights
Yonago Acta medica 編集委員会
Citation
Yonago Acta medica. 2006, 49(1), 39-47
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English