File
Authors
Keywords
capillary gas chromatography
19-hydroxycholesterol
internal standard compound
quantitative determination
sterols
Abstract
Gas chromatography is able to detect many kinds of sterols simultaneously. In order to identify and estimate accurately many kinds of sterols in biological samples by gas chromatography, it is indispensable to add an internal standard compound into the analytical samples prior to the analysis. However, there is no report on a suitable internal standard compound for the determination of sterols. In this study, 19-hydroxycholesterol was proved to be a suitable internal standard compound for simultaneous and quantitative determination of several sterols in various analytical samples by capillary gas chromatography, including the quantitative determination of β-cholestanol in the human plasma. Moreover, the excellent condition of gas chromatographic analysis for quantitative determination of several sterols in various analytical samples was found.
Publisher
Tottori University Faculty of Medicine
Content Type
Journal Article
ISSN・ISBN
1346-8049
NCID
AA00892882
Journal Title
Yonago Acta medica
Current Journal Title
Yonago Acta medica
Volume
45
Issue
1
Start Page
27
End Page
33
Published Date
2002-03
Text Version
Publisher
Rights
Yonago Acta medica 編集委員会
Citation
Yonago Acta medica. 2002, 45(1), 27-33
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English