ID 433
フルテキストファイル
著者
Yoshioka Shizue Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Tatebe Shigeru Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Tokuyasu Naruo Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine 鳥取大学研究者総覧
Taniguchi Kenjiro Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Ogami Yoshimi Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Yamamoto Osamu Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Ashida Keigo Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine 鳥取大学研究者総覧 KAKEN研究者をさがす
Gomyo Yoshihito Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Kondo Akira Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine KAKEN研究者をさがす
Tsujitani Shunichi Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Ikeguchi Masahide Division of Surgical Oncology, Department of Surgery, Division of Urology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine KAKEN研究者をさがす
キーワード
apoptosis
gastric cancer
PI3K-Akt pathway
SN-38
抄録
SN-38, an active metabolite of a topoisomerase I inhibitor, CPT-11, exhibits a cytotoxic effect by inducing apoptosis in cancer cells. Phosphatidylinositol-3-OH kinase (PI3K)-Akt signaling is known to protect a variety of cells from apoptosis. The relationship between resistance to SN-38-induced apoptosis and the PI3K-Akt pathway in human gastric cancer cells is unknown. Here, we did an investigation using two gastric cancer cell lines, MKN1 and MKN45. Cell viability was determined by sodium 3'-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate (XTT) assay. Apoptosis was confirmed by fluorescence microscopy using Hoechst 33342 staining. Expression levels of phospho-Akt (pAkt) were determined by Western blotting. After being treated with SN-38, the populations of sub-G1 cells were induced by flow cytometry in 36.8% of MKN45 cells more frequently than in 13.5% of MKN1 cells. SN-38 inhibited the expression of pAkt dose-dependently in MKN45 cells, but not in MKN1 cells. In MKN1 cells, an additional pretreatment with the PI3K inhibitor, LY294002, led to the inhibition of pAkt expression and induced apoptosis. The results suggested that SN-38 induces apoptosis by decreasing PI3K-Akt survival signaling, the anti-apoptotic signals, in human gastric cancer cells. Akt inhibitor might be a useful anti-tumor agent in combination with CPT-11.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN・ISBN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
48
1
開始ページ
7
終了ページ
16
発行日
2005-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2005, 48(1), 7-16
部局名
医学部・医学系研究科・医学部附属病院
言語
英語