フルテキストファイル
著者
Kishimoto Takuji Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine KAKEN研究者をさがす
Kaetsu Akihiko Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine KAKEN研究者をさがす
Osaki Yoneatsu Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine 鳥取大学研究者総覧 KAKEN研究者をさがす
Okamoto Mikizoh Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine KAKEN研究者をさがす
Nagai Mariya Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine
Kurosawa Yoichi Division of Health Administration and Promotion, Department of Social Medicine, Tottori University Faculty of Medicine 鳥取大学研究者総覧 KAKEN研究者をさがす
Yoshida Soichi Division of Reproductive-Perinatal Medicine and Gynecologic Oncology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
キーワード
gene expression
nitric oxide synthase
polymorphism
vascular endothelial cell
抄録
The relationship between cardiovascular disease and Glu298Asp polymorphism of nitric oxide synthase (eNOS) gene in vascular endothelial cells is controversial due to conflicting results. To demonstrate the effects of Glu298Asp polymorphism of the eNOS gene on eNOS gene expression by excluding the effects of various confounding factors, eNOS mRNA and protein expressions were measured using cultured human umbilical vein endothelial cells (HUVECs) according to genotypes of Glu298Asp polymorphism of the eNOS gene. eNOS mRNA expression was measured by real-time PCR/reverse trasnscription-PCR. eNOS protein expression was measured by an immunoassay method. HUVECs with Glu298Asp tended to show higher eNOS mRNA and protein expressions than those with Glu298Glu, but the difference was not significant. These results suggest that the genotypic difference of Glu298Glu and Glu298Asp has no effect on eNOS mRNA and protein expressions.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN・ISBN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
49
1
開始ページ
59
終了ページ
62
発行日
2006-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2006, 49(1), 59-62
部局名
医学部・医学系研究科・医学部附属病院
言語
英語