フルテキストファイル
著者
Fujita Seiya Department of chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Matsuura Kazunori Department of chemistry and Biotechnology, Graduate School of Engineering, Tottori University 鳥取大学研究者総覧 KAKEN研究者をさがす
抄録
In order to construct artificial viral capsids bearing complementary dimeric coiled-coils on the surface, β-annulus peptide bearing a coiled-coil forming sequence at the C-terminus (β-annulus-coiled-coil-B) was synthesized by a native chemical ligation of β-annulus-SBn peptide with Cys-containing coiled-coil-B peptide. Dynamic light scattering (DLS) measurements and transmission electron microscope (TEM) images revealed that the β-annulus-coiled-coil-B peptide self-assembled into spherical structures of about 50 nm in 10 mM Tris-HCl buffer. Circular dichroism (CD) spectra indicated formation of the complementary coiled-coil structure on the spherical assemblies. Addition of 0.25 equivalent of complementary coiled-coil-A peptide to the β-annulus-coiled-coil-B peptide showed the formation of spherical assemblies of 46 ± 14 nm with grains of 5 nm at the surface, whereas addition of 1 equivalent of complementary coiled-coil-A peptide generated fibrous assemblies.
出版者
Royal Society of Chemistry
資料タイプ
学術雑誌論文
外部リンク
ISSN・ISBN
14770520
書誌ID
AA1168650X
掲載誌名
Organic & biomolecular chemistry
最新掲載誌名
Organic & biomolecular chemistry
15
23
開始ページ
5070
終了ページ
5077
発行日
2017-05-25
出版者DOI
著者版フラグ
著者版
著作権表記
© The Royal Society of Chemistry 2017
掲載情報
This research was published by Elsevier : Fujita S. and Matsuura K. Self-assembled artificial viral capsid bearing coiled-coils at the surface. Organic & biomolecular chemistry. 2017, 15, 5070-5077. doi: https://doi.org/10.1039/C7OB00998D
部局名
工学部・工学研究科
言語
英語