フルテキストファイル
著者
Ogura Yoshio Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, School of Medicine, Tottori University Faculty of Medicine
Takei Tsuyoshi Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, School of Medicine, Tottori University Faculty of Medicine
Yamaga Nobuo Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, School of Medicine, Tottori University Faculty of Medicine
Yamada Kazuo Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, School of Medicine, Tottori University Faculty of Medicine
Uchida Kiyohisa Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, School of Medicine, Tottori University Faculty of Medicine
キーワード
Escherichia coli
cholic acid
glycocholic acid
3α,12α-dihydroxy-7-oxo-5β-cholanoic acid
in vitro biotransformation
抄録
The oxidation/reduction reactions of bile acids by Escherichia coli (E. coli) K-12 were examined in both Davis and brain-heart infusion (BHI) media under aerobic and anaerobic conditions. The pH in the Davis medium changed by almost the same amount, around pH 6.5?7.0 in both aerobic and anaerobic cultures, but the pH in the BHI medium was different in both cultures, that is, about pH 9.0 in the aerobic culture but only about 6.5 in the anaerobic culture. The growth curve of E. coli in the Davis medium showed a similar pattern in both conditions. Cholic acid (CA) was oxidized to 3α12α-dihydroxy-7-oxo-5β-cholanoic acid (3α12α7=O) in both cultures, but the reaction in the anaerobic culture was somewhat slower than that in the aerobic culture. On the other hand, reduction of 3α12α7=O to CA did not occur in the aerobic culture, but about 10% reduction was observed in the anaerobic culture after 4 days. These data suggest that the oxidation/reduction reaction of E. coli was oxidative in aerobic culture but reductive in anaerobic culture and these characteristics were not due to the changes in the pH of the medium. The reactions of CA and glycocholic acid to crude 7α-HSDH prepared from E. coli were examined and it was found that both free and conjugated CA as a substrate for the 7α-HSDH showed similar Km values.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN・ISBN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
49
2
開始ページ
71
終了ページ
75
発行日
2006-06
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2006, 49(3), 71-75
部局名
医学部・医学系研究科・医学部附属病院
言語
英語