ID 12762
File
Title Transcription
イガン フクスイチュウ エクソソーム ニ ソンザイスル microRNA ノ リンショウオウヨウ ニ ムケタ キソテキケンキュウ
Title Alternative
Clinical application of exosomal microRNAs in peritoneal fluids of gastric cancer patients with peritoneal metastases
Authors
Miyatani, Kozo Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine Researchers DB
Matsumi, Yoshiaki Division of Chemical Biology, Technical Department, Tottori University
Sawata, Shohei Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine
Makinoya, Masahiro Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine
Miyauchi, Wataru Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine
Shishido, Yuji Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine Researchers DB
Matsunaga, Tomoyuki Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine Researchers DB
Sakamoto, Teruhisa Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine Researchers DB
Hasegawa, Toshimichi Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine
Horie, Atsushi Laboratory of Electron Microscopy, Tottori University
Fujiwara, Yoshiyuki Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine,  Tottori University Faculty of Medicine Researchers DB KAKEN
Keywords
Gastric cancer
peritoneal metastasis
exosome
microRNA
biomarker
Abstract
The purpose of this study is to explore the exosomal microRNAs obtained from peritoneal fluids of gastric cancer patients, which are specific for peritoneal metastasis (PM) and could be a predictive marker of disease status in PM. Exosomes were isolated from peritoneal fluids of patients of gastric cancer with PM. Presence of isolated extracellular vesicles (EVs) from peritoneal fluid specimens were confirmed using a transmission electron microscope showed that many EVs were approximately 100 nm in diameter, which were equivalent to exosome fractions. We carried out RNA extraction and comprehensive array analysis of exosomal microRNAs and have identified that 86 microRNAs were significantly up- or down-regulated in all 4 specimens and that out of them, miR-323-3p was highly expressed in all 4 cases. In this way, we have established an experimental system for measuring exosomal microRNAs in peritoneal lavage specimens. Next, we plan to apply this analysis for additional peritoneal lavage specimens obtained from gastric cancer patients with or without PM and to identify specific microRNAs, which could be predictive biomarkers for the disease status of PM in gastric cancer.
Publisher
米子医学会
Content Type
Departmental Bulletin Paper
Link
ISSN
00440558
NCID
AN00247383
Journal Title
米子医学雑誌
Current Journal Title
The Journal of the Yonago Medical Association
Volume
72
Issue
1-4
Start Page
1
End Page
5
Published Date
2021-08-31
Text Version
Publisher
Rights
注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.
Citation
米子医学雑誌. 2021, 72(1-4), 1-5.
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
Japanese