ID 439
File
Authors
Keywords
apoptosis
ganciclovir
necrosis
thymidine kinase
Abstract
The tumoricidal effect of herpes simplex virus thymidine kinase (HSV-tk) gene transfer followed by ganciclovir (GCV) treatment has been demonstrated in relation to the bystander effect. We examined the mode of cell death after GCV treatment in three human oral squamous cell carcinoma (SCC) cell lines that had been infected with adenovirus possessing HSV-tk gene. Oral SCC cell lines displayed high susceptibility to HSV-tk/GCV treatment despite low transduction. Evidence suggests that apoptosis was not found by internucleosomal DNA fragmentation, cell cycle kinetics or gene expressions indicative of apoptosis. However, we observed different levels of expression of annexin V-positive/propidium iodide-negative cells in all the cell lines and occasional TUNEL-positive cells in one cell line. To address these controversial findings, we further confirmed the morphological phenotypes of cell death with semi-thin and ultra-thin sections, which revealed that the cells undergoing death were consistent with necrosis, i.e. swelling of cytoplasm and intracellular organs with membrane disruption. Furthermore, no apoptotic bodies were detected within cytoplasm of apparently intact cells. A nonapoptotic mechanism may play a central role in the HSV-tk/GCV-induced cell death in oral SCC cell lines.
Publisher
Tottori University Faculty of Medicine
Content Type
Journal Article
ISSN
1346-8049
NCID
AA00892882
Journal Title
Yonago Acta medica
Current Journal Title
Yonago Acta medica
Volume
48
Issue
3
Start Page
63
End Page
74
Published Date
2005-09
Text Version
Publisher
Rights
Yonago Acta medica 編集委員会
Citation
Yonago Acta medica. 2005, 48(3), 63-74
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English