ID 6558
File
Authors
Matsuo, Noriyuki Anatomy and Neuroscience, Osaka University Medical School / Dainippon Pharmaceutical Company Limited
Ogawa, Satoshi Anatomy and Neuroscience, Osaka University Medical School
Takagi, Tsutomu Molecular Neurobiology (Tanabe), Osaka University Medical School
Wanaka, Akio Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical College
Mori, Tetsuji Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical College Researchers DB KAKEN
Matsuyama, Tomohiro Anatomy and Neuroscience, Osaka University Medical School
J. Pinsky, David Department of Physiology and Cellular Biophysics and Surgery, College of Physicians and Surgeons, Columbia University
M. Stern, David Department of Physiology and Cellular Biophysics and Surgery, College of Physicians and Surgeons, Columbia University
Tohyama, Masaya Anatomy and Neuroscience, Osaka University Medical School
Abstract
To elucidate the role of astrocytes in the stress response of the central nervous system to ischemia, early gene expression was evaluated in cultured rat astrocytes subjected to hypoxia/reoxygenation. Using differential display, a novel putative vesicle transport-related factor (RA410) was cloned from reoxygenated astrocytes. Analysis of the deduced amino acid sequence showed RA410 to be composed of domains common to vesicle transport-related proteins of the Sec1/Unc18 family, including Sly1p and Sec1p (yeast), Rop (Drosophila), Unc18 (Caenorhabditis elegans), and Munc18 (mammalian), suggesting its possible role in vesicular transport. Northern analysis of normal rat tissues showed the highest expression of RA410 transcripts in testis. When astrocyte cultures were subjected to a period of hypoxia followed by reoxygenation, induction of RA410 mRNA was observed within 15 min of reoxygenation, reaching a maximum by 60 min. At the start of reoxygenation, the addition of diphenyl iodonium, an NADPH oxidase inhibitor, blocked in parallel astrocyte generation of reactive oxygen intermediates and expression of RA410 message. In contrast, cycloheximide did not affect RA410 mRNA levels, indicating that RA410 is an immediate-early gene in the setting of reoxygenation. Using polyclonal antibody raised against an RA410-derived synthetic peptide, Western blotting of lysates from reoxygenated astrocytes displayed an immunoreactive band of ≈70 kDa, the expression of which followed induction of the mRNA. Fractionation of astrocyte lysates on sucrose gradients showed RA410 antigen to be predominantly in the plasma membrane. Immunoelectron microscopic analysis demonstrated RA410 in large vesicles associated with the Golgi, but not in the Golgi apparatus itself, consistent with its participation in post-Golgi transport. Consistent with thesein vitro data, RA410 expression was observed in rat brain astrocytes following transient occlusion of the middle cerebral artery. These data provide insight into a new protein (RA410) that participates in the ischemia-related stress response in astrocytes.
Publisher
The American Society for Biochemistry and Molecular Biology
Content Type
Journal Article
Link
ISSN
1083351X
NCID
AA1202441X
Journal Title
The journal of biological chemistry
Current Journal Title
The journal of biological chemistry
Volume
272
Issue
26
Start Page
16438
End Page
16444
Published Date
1997-06-27
Publisher-DOI
Text Version
Publisher
Rights
© the American Society for Biochemistry and Molecular Biology
Citation
This research was originally published in the Journal of Biological Chemistry. Matsuo, Noriyuki, et al. Cloning of a putative vesicle transport-related protein, RA410, from cultured rat astrocytes and its expression in ischemic rat brain. J. Biol. Chem. 1997; 272:16438-16444. © the American Society for Biochemistry and Molecular Biology
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English