ID | 9460 |
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Authors |
Tsuchiya, Hiroyuki
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University
Researchers DB
KAKEN
Amisaki, Masataka
Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University
KAKEN
Takenaga, Ai
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University
Honjo, Soichiro
Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University
Researchers DB
Fujiwara, Yoshiyuki
Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University
Researchers DB
KAKEN
Shiota, Goshi
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University
Researchers DB
KAKEN
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Keywords | URI1
HBx
c-MYC
HBV-related hepatocellular carcinoma
E-box
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Abstract | Unconventional prefoldin RNA polymerase II subunit 5 interactor (URI1) has emerged as an oncogenic driver in hepatocellular carcinoma (HCC). Although the hepatitis B virus (HBV) represents the most common etiology of HCC worldwide, it is unknown whether URI1 plays a role in HBV-related HCC (HCC-B). In the present study, we investigated URI1 expression and its underlying mechanism in HCC-B tissues and cell lines. URI1 gene-promoter activity was determined by a luciferase assay. Human HCC-B samples were used for a chromatin immunoprecipitation assay. We found that c-MYC induced URI1 expression and activated the URI1 promoter through the E-box in the promoter region while the HBx protein significantly enhanced it. The positivity of URI1 expression was significantly higher in HCC-B tumor tissues than in non-HBV-related HCC tumor tissues, suggesting that a specific mechanism underlies URI1 expression in HCC-B. In tumor tissues from HCC-B patients, a significantly higher level of c-MYC was recruited to the E-box than in non-tumor tissues. These results suggest that HBx and c-MYC are involved in URI1 expression in HCC-B. URI1 expression may play important roles in the development and progression of HCC-B because HBx and c-MYC are well-known oncogenic factors in the virus and host, respectively.
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Publisher | MDPI
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Content Type |
Journal Article
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EISSN | 14220067
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Journal Title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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Current Journal Title |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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Volume | 20
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Issue | 22
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Published Date | 2019-11
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Text Version |
Publisher
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Rights | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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Citation | Tsuchiya Hiroyuki, Amisaki Masataka, Takenaga Ai, et al. HBx and c-MYC Cooperate to Induce URI1 Expression in HBV-Related Hepatocellular Carcinoma. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2019. 20(22). doi:10.3390/ijms20225714
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Department |
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
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Language |
English
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Web of Science Key ut | WOS:000502786800196
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