ID 9460
File
Authors
Tsuchiya, Hiroyuki Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University Researchers DB KAKEN
Amisaki, Masataka Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University KAKEN
Takenaga, Ai Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University
Honjo, Soichiro Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University Researchers DB
Fujiwara, Yoshiyuki Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University Researchers DB KAKEN
Shiota, Goshi Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University Researchers DB KAKEN
Keywords
URI1
HBx
c-MYC
HBV-related hepatocellular carcinoma
E-box
Abstract
Unconventional prefoldin RNA polymerase II subunit 5 interactor (URI1) has emerged as an oncogenic driver in hepatocellular carcinoma (HCC). Although the hepatitis B virus (HBV) represents the most common etiology of HCC worldwide, it is unknown whether URI1 plays a role in HBV-related HCC (HCC-B). In the present study, we investigated URI1 expression and its underlying mechanism in HCC-B tissues and cell lines. URI1 gene-promoter activity was determined by a luciferase assay. Human HCC-B samples were used for a chromatin immunoprecipitation assay. We found that c-MYC induced URI1 expression and activated the URI1 promoter through the E-box in the promoter region while the HBx protein significantly enhanced it. The positivity of URI1 expression was significantly higher in HCC-B tumor tissues than in non-HBV-related HCC tumor tissues, suggesting that a specific mechanism underlies URI1 expression in HCC-B. In tumor tissues from HCC-B patients, a significantly higher level of c-MYC was recruited to the E-box than in non-tumor tissues. These results suggest that HBx and c-MYC are involved in URI1 expression in HCC-B. URI1 expression may play important roles in the development and progression of HCC-B because HBx and c-MYC are well-known oncogenic factors in the virus and host, respectively.
Publisher
MDPI
Content Type
Journal Article
Link
EISSN
14220067
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Current Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume
20
Issue
22
Published Date
2019-11
Publisher-DOI
Text Version
Publisher
Rights
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Citation
Tsuchiya Hiroyuki, Amisaki Masataka, Takenaga Ai, et al. HBx and c-MYC Cooperate to Induce URI1 Expression in HBV-Related Hepatocellular Carcinoma. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2019. 20(22). doi:10.3390/ijms20225714
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English
Web of Science Key ut
WOS:000502786800196