File
Authors
Keywords
bladder dysfunction
bladder outlet obstruction
L-arginine
rat
Abstract
Nitric oxide (NO) is thought to play an important role in the lower urinary tract. We therefore studied the effects of NO on bladder function in bladder outlet-obstructed rats. Twelve-week-old female Wistar rats had their bladder outlets partially obstructed by ligating the urethra over which a catheter was placed. Micturition parameters and urodynamic parameters were measured in 6 groups of rats: Group I, 1 week after sham operation; Group II, 1 week after surgical induction of bladder outlet obstruction; Group III, 150-mg/kg/day L-arginine injected intraperitoneally for 1 week after surgical induction of bladder outlet obstruction; Group IV, 6 weeks after sham operation; Group V, 6 weeks after surgical induction of bladder outlet obstruction and Group VI, 150-mg/kg/day L-arginine injected intraperitoneally for 6 weeks after surgical induction of bladder outlet obstruction. Moreover, the area density of smooth muscle versus connective tissue was determined in bladder specimens using color assisted computer image analysis in these groups. The number of micturitions per day of the 1- and 6-week obstructed rats significantly increased over that of the sham-operated rats. The number of micturitions per day of the obstructed rats treated with L-arginine for 1 and 6 weeks significantly decreased in comparison to that of the obstructed rats. In the urodynamic study, the maximum detrusor pressure of the obstructed rats for 1 and 6 weeks significantly increased over that of the sham-operated rats. Residual urine volume of the obstructed rats for 1 and 6 weeks significantly increased over that of the sham-operated rats. The maximum detrusor pressure and residual urine volume of the obstructed rats treated with L-arginine for 1 and 6 weeks significantly decreased over those of the obstructed rats. In the histological study, the area density of smooth muscle versus connective tissue of the 1- and 6-week obstructed rats significantly increased over that of the sham-operated rats. Histological changes in the 1- and 6-week obstructed rats were prevented by treatment with L-arginine. These results indicate that L-arginine has a beneficial effect on bladder dysfunction in bladder outlet-obstructed rats. This might be due to L-arginine's influence in increasing NO levels and the rate of blood flow in the obstructed bladder.
Publisher
Tottori University Faculty of Medicine
Content Type
Journal Article
ISSN・ISBN
1346-8049
NCID
AA00892882
Journal Title
Yonago Acta medica
Current Journal Title
Yonago Acta medica
Volume
45
Issue
1
Start Page
9
End Page
18
Published Date
2002-03
Text Version
Publisher
Rights
Yonago Acta medica 編集委員会
Citation
Yonago Acta medica. 2002, 45(1), 9-18
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English