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Authors | |
Keywords | B7-H1
B7-H4
gastric cancer
macrophage
monocyte
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Abstract | Expression of the costimulatory molecule B7-H4, a member of the inhibitory B7 family, has been reported to be upregulated on tumor-associated macrophages, and this overexpression may be involved in immune evasion in cancer patients. The present study was designed to investigate B7-H4 expression on monocytes/macrophages and its relationship with immune evasion in gastric cancer patients. B7-H4 expression on circulating monocytes and tumor-associated macrophages was evaluated by multicolor flow cytometry. Carboxyfluorescein succinimidyl ester proliferation assays and quantitative interferon-gamma enzyme-linked immunosorbent assays were carried out to determine the inhibitory effect of B7-H4+ monocytes on CD4+ T cells. B7-H4 expression on circulating monocytes was upregulated and these B7-H4+ monocytes showed immunosuppressive properties. B7-H4 expression was closely related to the depth of invasion, as well as the presence of lymphatic and venous invasion. There were significant correlations between B7-H4 expression and B7-H1 or HLA-DR expression on monocytes/macrophages in gastric cancer patients. B7-H4 expression was remarkably upregulated in gastric cancer tissues compared with peripheral blood samples. Complete surgical removal of the tumor decreased B7-H4 expression on circulating monocytes from gastric cancer patients. Cocultures of gastric cancer cell lines and monocytes led to upregulation of B7-H4 expression on monocytes. Increased B7-H4 expression on circulating monocytes and tumor-associated macrophages may be one of the key mechanisms responsible for immune evasion by tumors in gastric cancer.
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Publisher | Tottori University Faculty of Medicine
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Content Type |
Journal Article
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Link | |
ISSN | 1346-8049
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NCID | AA00892882
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Journal Title | Yonago Acta medica
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Current Journal Title |
Yonago Acta medica
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Volume | 54
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Issue | 1
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Start Page | 1
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End Page | 10
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Published Date | 2011-03
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Text Version |
Publisher
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Rights | Yonago Acta medica 編集委員会
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Citation | Yonago Acta medica. 2011, 54(1), 1-10
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Language |
English
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