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| Title Transcription | イガン フクスイチュウ エクソソーム ニ ソンザイスル microRNA ノ リンショウオウヨウ ニ ムケタ キソテキケンキュウ
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| Title Alternative | Clinical application of exosomal microRNAs in peritoneal fluids of gastric cancer patients with peritoneal metastases
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| Authors |
Miyatani, Kozo
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine
Researchers DB
Matsumi, Yoshiaki
Division of Chemical Biology, Technical Department, Tottori University
Sawata, Shohei
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Makinoya, Masahiro
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Miyauchi, Wataru
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Shishido, Yuji
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Researchers DB
Matsunaga, Tomoyuki
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Researchers DB
Sakamoto, Teruhisa
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Researchers DB
Hasegawa, Toshimichi
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Horie, Atsushi
Laboratory of Electron Microscopy, Tottori University
Fujiwara, Yoshiyuki
Division of Gastrointestinal and Pediatric Surgery, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
Researchers DB
KAKEN
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| Keywords | Gastric cancer
peritoneal metastasis
exosome
microRNA
biomarker
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| Abstract | The purpose of this study is to explore the exosomal microRNAs obtained from peritoneal fluids of gastric cancer patients, which are specific for peritoneal metastasis （PM） and could be a predictive marker of disease status in PM. Exosomes were isolated from peritoneal fluids of patients of gastric cancer with PM. Presence of isolated extracellular vesicles （EVs） from peritoneal fluid specimens were confirmed using a transmission electron microscope showed that many EVs were approximately 100 nm in diameter, which were equivalent to exosome fractions. We carried out RNA extraction and comprehensive array analysis of exosomal microRNAs and have identified that 86 microRNAs were significantly up- or down-regulated in all 4 specimens and that out of them, miR-323-3p was highly expressed in all 4 cases. In this way, we have established an experimental system for measuring exosomal microRNAs in peritoneal lavage specimens. Next, we plan to apply this analysis for additional peritoneal lavage specimens obtained from gastric cancer patients with or without PM and to identify specific microRNAs, which could be predictive biomarkers for the disease status of PM in gastric cancer.
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| Publisher | 米子医学会
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| Content Type |
Departmental Bulletin Paper
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| Link | |
| ISSN | 00440558
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| NCID | AN00247383
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| Journal Title | 米子医学雑誌
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| Current Journal Title |
The Journal of the Yonago Medical Association
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| Volume | 72
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| Issue | 1-4
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| Start Page | 1
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| End Page | 5
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| Published Date | 2021-08-31
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| Text Version |
Publisher
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| Rights | 注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.
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| Citation | 米子医学雑誌. 2021, 72(1-4), 1-5.
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| Department |
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
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| Language |
Japanese
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