File
Authors
Murata, Akihiko Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University Researchers DB KAKEN
Hikosaka, Mari Department of Stem Cell and Developmental Biology, Mie University Graduate School of Medicine
Yoshino, Miya Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University Researchers DB
Zhou, Lan Department of Pathology, Case Western Reserve University
Hayashi, Shin-ichi Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University Researchers DB KAKEN
Keywords
Delta-like
Jagged
Abstract
Kit/CD117 plays a crucial role in the cell–cell and cell–matrix adhesion of mammalian mast cells (MCs); however, it is unclear whether other adhesion molecule(s) perform important roles in the adhesion of MCs. In the present study, we show a novel Kit-independent adhesion mechanism of mouse cultured MCs mediated by Notch family members. On stromal cells transduced with each Notch ligand gene, Kit and its signaling become dispensable for the entire adhesion process of MCs from tethering to spreading. The Notch-mediated spreading of adherent MCs involves the activation of signaling via phosphatidylinositol 3-kinases and mitogen-activated protein kinases, similar to Kit-mediated spreading. Despite the activation of the same signaling pathways, while Kit supports the adhesion and survival of MCs, Notch only supports adhesion. Thus, Notch family members are specialized adhesion molecules for MCs that effectively replace the adhesion function of Kit in order to support the interaction of MCs with the surrounding cellular microenvironments.
Publisher
Oxford University Press
Content Type
Journal Article
Link
ISSN
09538178
EISSN
14602377
Journal Title
INTERNATIONAL IMMUNOLOGY
Current Journal Title
INTERNATIONAL IMMUNOLOGY
Volume
31
Issue
2
Start Page
69
End Page
79
Published Date
2019-02
Publisher-DOI
Text Version
Author
Rights
This is a pre-copyedited, author-produced version of an article accepted for publication in INTERNATIONAL IMMUNOLOGY following peer review. The version of record Akihiko Murata, Mari Hikosaka, Miya Yoshino, Lan Zhou, Shin-Ichi Hayashi, Kit-independent mast cell adhesion mediated by Notch, International Immunology, Volume 31, Issue 2, February 2019, Pages 69–79, https://doi.org/10.1093/intimm/dxy067 is available online at: https://doi.org/10.1093/intimm/dxy067
Citation
Murata Akihiko, Hikosaka Mari, Yoshino Miya, et al. Kit-independent mast cell adhesion mediated by Notch. INTERNATIONAL IMMUNOLOGY. 2019. 31(2). 69-79. doi:10.1093/intimm/dxy067
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English
Web of Science Key ut
WOS:000461515600002