dms10_s13098-018-0351-4.pdf 1.34 MB
Sumi, Keisuke Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University Researchers DB
Okura, Tsuyoshi Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University Researchers DB KAKEN
Fujioka, Yohei Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University Researchers DB KAKEN
Kato, Masahiko Division of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University Researchers DB KAKEN
Imamura, Takeshi Division of Molecular Pharmacology, Faculty of Medicine, Tottori University Researchers DB KAKEN
Taniguchi, Shin-ichi Department of Regional Medicine, Faculty of Medicine, Tottori University Researchers DB KAKEN
Background: In mitochondrial diabetes, apoptosis of β-cells caused by mitochondrial stress plays an important role in impaired insulin secretion. Several studies have reported that coenzyme Q10 (CoQ10) has therapeutic effects on mitochondrial diabetes, but no reports have examined the fundamental effectiveness or mechanism of CoQ10 in mitochondrial diabetes. We previously reported in a Japanese article that CoQ10 has protective effects on pancreatic β-cells against mitochondrial stress using mouse pancreatic β-cell line MIN6 and staurosporine (STS). Here, we report that CoQ10 protects MIN6 cells against apoptosis caused by STS and describe the more detailed apoptotic cascade. Methods: Apoptosis of MIN6 cells was induced by 0.5 μM STS treatment for specific periods with or without 30 μM CoQ10. The apoptosis cascade in MIN6 cells was then investigated using WST-8 assays, annexin-V staining, western blotting, and DNA degradation analysis. Results: Sixteen hours of 0.5 μM STS treatment led to 47% cell viability, but pretreatment with 30 μM CoQ10 resulted in significantly higher viability of 76% (P < 0.01). CoQ10 also prevented translocation of phosphatidylserine from the inner leaflet to the outer leaflet of the cell membrane. CoQ10 prevented cytochrome c release from mitochondria and activation of caspase-3. Conclusion: We concluded that CoQ10 protects pancreatic β-cells through anti-apoptotic effects against STS treatment.
BioMed Central Ltd.
DIABETOLOGY & METABOLIC SYNDROME
|Current Journal Title||
DIABETOLOGY & METABOLIC SYNDROME
© The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/publi cdoma in/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Sumi Keisuke, Okura Tsuyoshi, Fujioka Youhei, et al. Coenzyme Q10 suppresses apoptosis of mouse pancreatic beta-cell line MIN6. DIABETOLOGY & METABOLIC SYNDROME. 2018. 10. doi:10.1186/s13098-018-0351-4
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
|Web of Science Key ut||