File
Authors
Keywords
circular dichroism
GATA-2
transcription factor
Zn finger
Abstract
The C-terminal zinc finger domain of GATA-2 transcription factor from Rattus norvegicus has been expressed and purified to elucidate its secondary structure using circular dichroism spectroscopy. Circular dichroism spectra showed that native GATA-2 C-terminal domain of (Cys)4 type zinc finger has 12% α-helix, 36% β-sheet and 52% random coil content. The estimated structure was compared with predicted structures by sequence based prediction software SOPMA and found to be similar. Furthermore, the effect of pH on the secondary structure of GATA-2 C-finger was examined. This study provides an insight into the understanding of the structure and function of transcription factor GATA-2.
Publisher
Tottori University Faculty of Medicine
Content Type
Journal Article
ISSN
1346-8049
NCID
AA00892882
Journal Title
Yonago Acta medica
Current Journal Title
Yonago Acta medica
Volume
53
Issue
1
Start Page
25
End Page
28
Published Date
2010-03
Text Version
Publisher
Rights
Yonago Acta medica 編集委員会
Citation
Yonago Acta medica. 2010, 53(1), 25-28
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English