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Authors
Nakamura, Yoko Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Sato, Yuki Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Inaba, Hiroshi Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University Researchers DB KAKEN
Iwasaki, Takashi Department of Bioresources Science, Graduate School of Agricultural Sciences, Tottori University Researchers DB KAKEN
Matsuura, Kazunori Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University Researchers DB KAKEN
Keywords
mRNA
poly(A) tail
artificial viral capsid
encapsulation
nanocapsule
self-assembly
β-annulus peptide
peptide-DNA conjugate
Abstract
Messenger RNA (mRNA) drugs have attracted considerable attention as promising tools with many therapeutic applications. The efficient delivery of mRNA drugs using non-viral materials is currently being explored. We demonstrate a novel concept where mCherry mRNA bearing a poly(A) tail is encapsulated into capsids co-assembled from viral β-annulus peptides bearing a 20-mer oligothymine (dT20) at the N-terminus and unmodified peptides via hybridization of dT20 and poly(A). Dynamic light scattering measurements and transmission electron microscopy images of the mRNA-encapsulated capsids show the formation of spherical assemblies of approximately 50 nm. The encapsulated mRNA shows remarkable ribonuclease resistance. Further, modification by a cell-penetrating peptide (His16) on the capsid enables the intracellular expression of mCherry of encapsulated mRNA.
Publisher
MDPI
Content Type
Journal Article
Link
EISSN
20763417
Journal Title
APPLIED SCIENCES
Volume
10
Issue
22
Published Date
2020-11
Publisher-DOI
Text Version
Publisher
Rights
(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Citation
Nakamura Yoko, Sato Yuki, Inaba Hiroshi, et al. Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate and the Poly(A) Tail of mRNA. APPLIED SCIENCES-BASEL. 2020. 10(22). doi:10.3390/app10228004
Department
Faculty of Engineering/Graduate School of Engineering
Language
English
Web of Science Key ut
WOS:000594917600001