File
Authors
Suzuki, Hirokazu Faculty of Engineering, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University Researchers DB KAKEN
Taketani, Tatsunari Department of Engineering, Graduate School of Sustainability Science, Tottori University
Tanabiki, Misaki Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Ohara, Misaki Department of Engineering, Graduate School of Sustainability Science, Tottori University
Kobayashi, Jyumpei Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Ohshiro, Takashi Faculty of Engineering, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University Researchers DB KAKEN
Keywords
extra-cytoplasmic function sigma factor
IS4
IS701
IS1634
ISLre2
stress-induced transposition
thermophile
transposable element
Abstract
Geobacillus kaustophilus HTA426 is a thermophilic bacterium whose genome harbors numerous insertion sequences (IS). This study was initially conducted to generate mutant genes for thermostable T7 RNA polymerase in G. kaustophilus; however, relevant experiments unexpectedly identified that the organism transposed multiple IS elements and produced derivative cells that expressed a silent gene via transposition. The transposed elements were diverse and included members of the IS4, IS701, IS1634, and ISLre2 families. The transposition was relatively active at elevated temperatures and generated 4–9 bp of direct repeats at insertion sites. Transposition was more frequent in proliferative cells than in stationary cells but was comparable between both cells when sigX, which encodes an extra-cytoplasmic function sigma factor, was forcibly expressed. Southern blot analysis indicated that IS transposition occurred under growth inhibitory conditions by diverse stressors; however, IS transposition was not detected in cells that were cultured under growth non-inhibitory conditions. These observations suggest that G. kaustophilus enhances IS transposition via sigX-dependent stress responses when proliferative cells were prevented from active propagation. Considering Geobacillus spp. are highly adaptive bacteria that are remarkably distributed in diverse niches, it is possible that these organisms employ IS transposition for environmental adaptation via genetic diversification. Thus, this study provides new insights into adaptation strategies of Geobacillus spp. along with implications for strong codependence between mobile genetic elements and highly adaptive bacteria for stable persistence and evolutionary diversification, respectively. This is also the first report to reveal active IS elements at elevated temperatures in thermophiles and to suggest a sigma factor that governs IS transposition.
Publisher
Frontiers Media
Content Type
Journal Article
Link
ISSN
1664302X
Journal Title
FRONTIERS IN MICROBIOLOGY
Volume
12
Published Date
2021-03-24
Publisher-DOI
Text Version
Publisher
Rights
(C) 2021 Suzuki, Taketani, Tanabiki, Ohara, Kobayashi and Ohshiro. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Citation
Suzuki Hirokazu, Taketani Tatsunari, Tanabiki Misaki, et al. Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426. FRONTIERS IN MICROBIOLOGY. 2021. 12. doi:10.3389/fmicb.2021.650461
Department
Faculty of Engineering/Graduate School of Engineering
Language
English
Web of Science Key ut
WOS:000637302700001