File
Authors
Ozaki, H. Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University / Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University Researchers DB KAKEN
Yonehara, K. Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University
Murase, T. Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University / Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University Researchers DB KAKEN
Keywords
avian pathogenic Escherichia coli
chicken
colibacillosis
embryo lethality assay
infection model
virulence
Abstract
To evaluate the virulence of avian pathogenic Escherichia coli (APEC) isolates obtained from colibacillosis cases associated with pericarditis, perihepatitis, and salpingitis, the embryo lethality assay and experimental infection model in chicks were used in this study. According to the established criteria based on mortality in the embryo lethality assay for evaluating the virulence of E. coli isolates, 23 of the 26 APEC isolates associated with pericarditis and perihepatitis and 8 of the 20 isolates associated with salpingitis were found to be virulent. Isolate D137, which had been obtained from a case with pericarditis and perihepatitis and had an embryo mortality of 92%, and isolate D445, which had been obtained from a case with pericarditis and perihepatitis and had an embryo mortality of 17%, were used for the experimental infection. Four of the five 11-day-old chickens inoculated through the air sac with isolate D137 died 1 day postinoculation, and the challenge strain was recovered from the air sac, pericardial sac, or liver; however, colibacillosis lesions were found in only one of the five birds postmortem. All five chicks inoculated with isolate D445 survived for 7 days postinoculation and exhibited airsacculitis or pericarditis lesions at 7 days postinoculation; the challenge strain was not recovered from the lesions postmortem. The results obtained in this study suggest that the different APEC isolates tested cause illness in chickens through distinct pathogenesis.
Publisher
BioOne
Content Type
Journal Article
Link
ISSN
00052086
EISSN
19384351
Journal Title
AVIAN DISEASES
Volume
62
Issue
2
Start Page
233
End Page
236
Published Date
2018-06
Publisher-DOI
Text Version
None
Rights
(C)2018 the American Association of Avian Pathologists
Citation
Ozaki H., Yonehara K., Murase T., et al. Virulence of Escherichia coli Isolates Obtained from Layer Chickens with Colibacillosis Associated with Pericarditis, Perihepatitis, and Salpingitis in Experimentally Infected Chicks and Embryonated Eggs. AVIAN DISEASES. 2018. 62(2). 233-236. doi:10.1637/11685-060717-ResNote.1
Department
Faculty of Agriculture/Graduate School of Agriculture
Language
English
Web of Science Key ut
WOS:000436414700014