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Authors
Yabuta, Yukinori Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University Researchers DB KAKEN
Nagata, Ryuta Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Aoki, Yuka Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Kariya, Ayumi Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Wada, Kousuke Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Yanagimoto, Ayako Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Hara, Hiroka Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University
Bito, Tomohiro Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University Researchers DB KAKEN
Okamoto, Naho The United Graduate School of Agricultural Sciences, Tottori University
Yoshida, Shinichi Electronic and Organic Material Laboratory, Tottori Institute of Industrial Technology
Ishihara, Atsushi Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University Researchers DB KAKEN
Watanabe, Fumio Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University Researchers DB KAKEN
Keywords
L-ascorbate (AsA)
antioxidant
biosynthetic pathway
Caenorhabditis elegans
reactive oxygen species
redox
Abstract
Ascorbate (AsA) is required as a cofactor and is widely distributed in plants and animals. Recently, it has been suggested that the nematode Caenorhabditis elegans also synthesizes AsA. However, its biosynthetic pathway is still unknown. To further understand AsA biosynthesis in C. elegans, we analyzed the incorporation of the 13C atom into AsA using gas chromatography-mass spectrometry (GC-MS) in worms fed with D-Glc (1-13C)-labeled Escherichia coli. GC-MS analysis revealed that AsA biosynthesis in C. elegans, similarly to that in mammalian systems, involves carbon skeleton rearrangement. The addition of L-gulono-1,4-lactone, an AsA precursor in the mammalian pathway, significantly increased AsA level in C. elegans, whereas the addition of L-galactono-1,4-lactone, an AsA precursor in the plant and Euglena pathway, did not affect AsA level. The suppression of E03H4.3 (an ortholog of gluconolactonase) or the deficiency of F54D5.12 (an ortholog of L-gulono-1,4-lactone oxidase) significantly decreased AsA level in C. elegans. Although N2- and AsA-deficient F54D5.12 knockout mutant worm (tm6671) morphologies and the ratio of collagen to non-collagen protein did not show any significant differences, the mutant worms exhibited increased malondialdehyde levels and reduced lifespan compared with the N2 worms. In conclusion, our findings indicate that the AsA biosynthetic pathway is similar in C. elegans and mammals.
Publisher
MDPI
Content Type
Journal Article
Link
EISSN
22181989
Journal Title
METABOLITES
Volume
10
Issue
8
Start Page
334
Published Date
2020-08
Publisher-DOI
Text Version
Publisher
Rights
(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Citation
Yabuta Yukinori, Nagata Ryuta, Aoki Yuka, et al. L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans. METABOLITES. 2020. 10(8). doi:10.3390/metabo10080334
Department
Faculty of Agriculture/Graduate School of Agriculture
Language
English
Web of Science Key ut
WOS:000564136300001