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Authors
Hirahara, Yukie Department of Anatomy and Cell Science, Kansai Medical University
Wakabayashi, Taketoshi Department of Anatomy and Cell Science, Kansai Medical University
Mori, Tetsuji Department of Anatomy and Cell Science, Kansai Medical University / School of Health Science, Faculty of Medicine, Tottori University Researchers DB KAKEN
Koike, Taro Department of Anatomy and Cell Science, Kansai Medical University
Yao, Ikuko Department of Optical Imaging, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Hamamatsu University School of Medicine
Tsuda, Masayuki The Division of Laboratory Animal Science, Science Research Center, Kochi University Medical School
Honke, Koichi Department of Biochemistry, Kochi University Medical School
Gotoh, Hitoshi Department of Biology, Kyoto Prefectural University of Medicine
Ono, Katsuhiko Department of Biology, Kyoto Prefectural University of Medicine
Yamada, Hisao Department of Anatomy and Cell Science, Kansai Medical University
Keywords
fatty acid chain
HSO3‐3‐galactosylceramide
imaging mass spectrometry
myelin
pro‐oligodendroblast
Abstract
HSO3‐3‐galactosylceramide (Sulfatide) species comprise the major glycosphingolipid components of oligodendrocytes and myelin and play functional roles in the regulation of oligodendrocyte maturation and myelin formation. Although various sulfatide species contain different fatty acids, it is unclear how these sulfatide species affect oligodendrogenesis and myelination. The O4 monoclonal antibody reaction with sulfatide has been widely used as a useful marker for oligodendrocytes and myelin. However, sulfatide synthesis during the pro‐oligodendroblast stage, where differentiation into the oligodendrocyte lineage has already occurred, has not been examined. Notably, this stage comprises O4‐positive cells. In this study, we identified a sulfatide species from the pro‐oligodendroblast‐to‐myelination stage by imaging mass spectrometry. The results demonstrated that short‐chain sulfatides with 16 carbon non‐hydroxylated fatty acids (C16) and 18 carbon non‐hydroxylated fatty acids (C18) or 18 carbon hydroxylated fatty acids (C18‐OH) existed in restricted regions of the early embryonic spinal cord, where pro‐oligodendroblasts initially appear, and co‐localized with Olig2‐positive pro‐oligodendroblasts. C18 and C18‐OH sulfatides also existed in isolated pro‐oligodendroblasts. C22‐OH sulfatide became predominant later in oligodendrocyte development and the longer C24 sulfatide was predominant in the adult brain. Additionally, the presence of each sulfatide species in a different area of the adult brain was demonstrated by imaging mass spectrometry at an increased lateral resolution. These findings indicated that O4 recognized sulfatides with short‐chain fatty acids in pro‐oligodendroblasts. Moreover, the fatty acid chain of the sulfatide became longer as the oligodendrocyte matured. Therefore, individual sulfatide species may have unique roles in oligodendrocyte maturation and myelination.
Publisher
International Society for Neurochemistry
Content Type
Journal Article
Link
ISSN
14714159
NCID
AA11628230
Journal Title
Journal of neurochemistry
Volume
140
Issue
3
Start Page
435
End Page
450
Original Article
Published Date
2016-11-12
Publisher-DOI
Text Version
Author
Rights
© 2016 International Society for Neurochemistry
Citation
This is the peer reviewed version of the following article: Hirahara, Y., Wakabayashi, T., Mori, T., Koike, T., Yao, I., Tsuda, M., Honke, K., Gotoh, H., Ono, K. and Yamada, H. (2017), Sulfatide species with various fatty acid chains in oligodendrocytes at different developmental stages determined by imaging mass spectrometry. J. Neurochem., 140: 435-450. doi:10.1111/jnc.13897, which has been published in final form at https://doi.org/10.1111/jnc.13897. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English