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Authors
Takamori, Yasuharu Department of Anatomy and Cell Science, Kansai Medical University
Hirahara, Yukie Department of Anatomy and Cell Science, Kansai Medical University
Wakabayashi, Taketoshi Department of Anatomy and Cell Science, Kansai Medical University
Mori, Tetsuji Department of Anatomy and Cell Science, Kansai Medical University / Faculty of Medicine, Tottori University Researchers DB KAKEN
Koike, Taro Department of Anatomy and Cell Science, Kansai Medical University
Kataoka, Yosky Laboratory for Cellular Function Imaging, RIKEN Center for Biosystems Dynamics Research / Multi-Modal Microstructure Analysis Unit, RIKEN-JEOL Collaboration Center
Tamura, Yasuhisa Laboratory for Cellular Function Imaging, RIKEN Center for Biosystems Dynamics Research / Multi-Modal Microstructure Analysis Unit, RIKEN-JEOL Collaboration Center
Kurebayashi, Shuji Department of Anatomy and Cell Science, Kansai Medical University / Department of School Education Research, Shizuoka University
Kurokawa, Kiyoshi Department of Anatomy and Cell Science, Kansai Medical University / Department of Human Health Science, Osaka international University
Yamada, Hisao Department of Anatomy and Cell Science, Kansai Medical University
Keywords
Lamins
Neurons
Glial cells
Adult rat
Cerebral cortex
Immunohistochemistry
Abstract
Lamins are type V intermediate filament proteins that are located beneath the inner nuclear membrane. In mammalian somatic cells, LMNB1 and LMNB2 encode somatic lamins B1 and B2, respectively, and the LMNA gene is alternatively spliced to generate somatic lamins A and C.

Mutations in lamin genes have been linked to many human hereditary diseases, including neurodegenerative disorders. Knowledge about lamins in the nervous system has been accumulated recently, but a precise analysis of lamin subtypes in glial cells has not yet been reported. In this study we investigated the composition of lamin subtypes in neurons, astrocytes, oligodendrocyte-lineage cells, and microglia in the adult rat cerebral cortex using an immunohistochemical staining method. Lamin A was not observed in neurons and glial cells. Lamin C was observed in astrocytes, mature oligodendrocytes and neurons, but not observed in oligodendrocyte progenitor cells. Microglia also did not stain positive for lamin C which differed from macrophages, with lamin C positive. Lamin B1 and B2 were observed in all glial cells and neurons. Lamin B1 was intensely positive in oligodendrocyte progenitor cells compared with other glial cells and neurons. Lamin B2 was weakly positive in all glial cells compared to neurons. Our current study might provide useful information to reveal how the onset mechanisms of human neurodegenerative diseases are associated with mutations in genes for nuclear lamin proteins.
Publisher
Elsevier Ltd
Content Type
Journal Article
Link
ISSN
24518301
Journal Title
IBRO Reports
Volume
5
Start Page
99
End Page
109
Research article
Published Date
2018-11-05
Publisher-DOI
Text Version
Publisher
Rights
© 2018 The Authors. Published by Elsevier Ltd on behalf of International Brain Research Organization. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Citation
TAKAMORI, Yasuharu, et al. Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex. IBRO reports, 2018, 5: 99-109.
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English