g54(1)_21.pdf 7.38 MB
Mori, Tetsuji Institute of Stem Cell Research, GSF‐National Research Center for Environment and Health Researchers DB KAKEN
Tanaka, Kohichi Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute Tokyo Medical and Dental University
Buffo, Annalisa Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute Tokyo Medical and Dental University
Kühn, Ralf Institute of Developmental Genetics, GSF-National Research Center for Environment and Health,
Wurst, Wolfgang Institute of Developmental Genetics, GSF-National Research Center for Environment and Health
Goetz, Magdalena GSF - National Research Center for Environment and Health / Department of Physiology, Ludwig-Maximilians University
inducible cre activity
Astrocytes are thought to play a variety of key roles in the adult brain, such as their participation in synaptic transmission, in wound healing upon brain injury, and adult neurogenesis. However, to elucidate these functions in vivo has been difficult because of the lack of astrocyte‐specific gene targeting. Here we show that the inducible form of Cre (CreERT2) expressed in the locus of the astrocyte‐specific glutamate transporter (GLAST) allows precisely timed gene deletion in adult astrocytes as well as radial glial cells at earlier developmental stages. Moreover, postnatal and adult neurogenesis can be targeted at different stages with high efficiency as it originates from astroglial cells. Taken together, this mouse line will allow dissecting the molecular pathways regulating the diverse functions of astrocytes as precursors, support cells, repair cells, and cells involved in neuronal information processing. © 2006 Wiley‐Liss, Inc.
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Copyright © 2006 Wiley‐Liss, Inc.
This is the peer reviewed version of the following article: Mori, T., Tanaka, K., Buffo, A., Wurst, W., Kühn, R. and Götz, M. (2006), Inducible gene deletion in astroglia and radial glia—A valuable tool for functional and lineage analysis. Glia, 54: 21-34. doi:10.1002/glia.20350, which has been published in final form at https://doi.org/10.1002/glia.20350. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital