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Title Alternative
CMV infection of trabecular meshwork cells
Authors
Shimizu, Daisuke Ophthalmology and Visual Science, Tottori University Faculty of Medicine / Department of Ophthalmology, Chiba University Graduate School of Medicine
Miyazaki, Dai Ophthalmology and Visual Science, Tottori University Faculty of Medicine Researchers DB KAKEN
Shimizu, Yumiko Ophthalmology and Visual Science, Tottori University Faculty of Medicine
Hosogai, Mayumi Department of Ophthalmology, Gunma University Graduate School of Medicine
Kosugi, Isao Regenerative and Infectious Pathology, Hamamatsu University, School of Medicine
Inoue, Yoshitsugu Ophthalmology and Visual Science, Tottori University Faculty of Medicine Researchers DB KAKEN
Keywords
cytomegalovirus
corneal endothelial cells
trabecular meshwork
secondary glaucoma
endotheliitis
Abstract
Purpose: Human cytomegalovirus (HCMV) infections can cause endotheliitis which is associated with an elevation of the intraocular pressure (IOP). However, the mechanism of the IOP elevation has not been determined. The purpose of this study was to determine whether HCMV strains which are capable of infecting corneal endothelial cells can also replicate, induce anti-viral responses, and can reorganize the actin cytoskeleton in trabecular meshwork cells.
Study design: Experimental study design
Methods: Cultured primary human trabecular meshwork cells (HTMCs) were infected with the Towne or TB40/E strains of HCMV. TB40/E is trophic for vascular endothelial and corneal endothelial cells. Real-time PCR, western blot, and fluorescent immunostaining have been used to determine whether HCMV-infected HTMCs will support the expression of viral mRNA and protein, allow viral replication, and elicit anti-viral host responses. We also determined whether lytic replication was present after an HCMV infection.
Results: HCMV infection led to the expression of viral mRNA and proteins of IE1, glycoprotein B(gB), and pp65. TB40/E infection induced interferon-β, a sign of host anti-viral immune response and MCP-1. Together with the induction of the regulators of actin cytoskeleton, myosin phosphatase Rho interacting protein (MPRIP) and monocyte chemotactic protein-1 (MCP-1), TB40/E induced a high level of expression of viral proteins, including IE1, gB, and pp65 as well as actin stress fiber formation, and achieved pathogenically high viral titers.
Conclusions: Human trabecular meshwork cells support the replication of endotheliotropic TB40/E strain of HCMV which indicates that this strain may have high virulence for trabecular meshwork.
Publisher
Springer Japan
Content Type
Journal Article
Link
ISSN・ISBN
0021-5155
NCID
AA00691177
Journal Title
Japanese Journal of Ophthalmology
Current Journal Title
Bulletin of the Tottori University Junior High School
Volume
62
Issue
6
Start Page
667
End Page
676
Published Date
2018-09-06
Publisher-DOI
Text Version
Author
Rights
© Japanese Ophthalmological Society 2018
Citation
Japanese Journal of Ophthalmology. 2018, 62(6), 667-676 This is a post-peer-review, pre-copyedit version of an article published in Japanese Journal of Ophthalmology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10384-018-0618-1
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English