File
Authors
Buffo, Annalisa Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
Rite, Inmaculada Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health / Institute of Physiology, University of Munich
Tripathi, Pratibha Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
Lepier, Alexandra Institute of Physiology, University of Munich
Colak, Dilek Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
Horn, Ana-Paula Department of Biochemistry, Federal University of Rio Grande do Sul
Mori, Tetsuji Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health Researchers DB KAKEN
Götz, Magdalena Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
Keywords
astrocytes
cell fate
cerebral cortex
stem cells
Abstract
Reactive gliosis is the universal reaction to brain injury, but the precise origin and subsequent fate of the glial cells reacting to injury are unknown. Astrocytes react to injury by hypertrophy and up-regulation of the glial-fibrillary acidic protein (GFAP). Whereas mature astrocytes do not normally divide, a subpopulation of the reactive GFAP+ cells does so, prompting the question of whether the proliferating GFAP+ cells arise from endogenous glial progenitors or from mature astrocytes that start to proliferate in response to brain injury. Here we show by genetic fate mapping and cell type-specific viral targeting that quiescent astrocytes start to proliferate after stab wound injury and contribute to the reactive gliosis and proliferating GFAP+ cells. These proliferating astrocytes remain within their lineage in vivo, while a more favorable environment in vitro revealed their multipotency and capacity for self-renewal. Conversely, progenitors present in the adult mouse cerebral cortex labeled by NG2 or the receptor for the platelet-derived growth factor (PDGFRα) did not form neurospheres after (or before) brain injury. Taken together, the first fate-mapping analysis of astrocytes in the adult mouse cerebral cortex shows that some astrocytes acquire stem cell properties after injury and hence may provide a promising cell type to initiate repair after brain injury.
Publisher
National Academy of Sciences
Content Type
Journal Article
Link
ISSN
10916490
NCID
AA11726874
Journal Title
PNAS : proceedings of the National Academy of Sciences of the United States of America
Current Journal Title
PNAS : proceedings of the National Academy of Sciences of the United States of America
Volume
105
Issue
9
Start Page
3581
End Page
3586
RESEARCH ARTICLE
Published Date
2008-03-04
Publisher-DOI
Text Version
Author
Rights
© 2008 by The National Academy of Sciences of the USA
Citation
Annalisa Buffo, Inmaculada Rite, Pratibha Tripathi, Alexandra Lepier, Dilek Colak, Ana-Paula Horn, Tetsuji Mori, Magdalena Götz. Origin and progeny of reactive gliosis: A source of multipotent cells in the injured brain. Proceedings of the National Academy of Sciences Mar 2008, 105 (9) 3581-3586; DOI: 10.1073/pnas.0709002105
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English