File
Authors
Mori, Tetsuji Department of Anatomy & Cell Science, Kansai Medical University Researchers DB KAKEN
Wakabayashi, Taketoshi Department of Anatomy & Cell Science, Kansai Medical University
Hirahara, Yukie Department of Anatomy & Cell Science, Kansai Medical University
Takamori, Yasuharu Department of Anatomy & Cell Science, Kansai Medical University
Kurokawa , Kiyoshi Department of Anatomy & Cell Science, Kansai Medical University
Yamada, Hisao Department of Anatomy & Cell Science, Kansai Medical University
Keywords
adult mouse SVZ
cell cycle
neurogenesis
precursor
seizure
Abstract
Adult neurogenesis in the subgranular zone of the hippocampus (SGZ) is enhanced by excess as well as mild neuronal excitation, such as chemoconvulsant‐induced brief seizures. Because most studies of neurogenesis after seizures have focused on the SGZ, the threshold of neuronal excitation required to enhance neurogenesis in the subventricular zone (SVZ) is not clear. Therefore, we examined the responses of SVZ precursors to brief generalized clonic seizures induced by a single administration of the chemoconvulsant pentylenetetrazole (PTZ). Cell cycle progression of precursors was analysed by systemic administration of thymidine analogues. We found that brief seizures immediately resulted in cell cycle retardation in the SVZ. However, the same effect was not seen in the SGZ. This initial cell cycle retardation in the SVZ was followed by enhanced cell cycle re‐entry after the first round of mitosis, leading to precursor pool expansion, but the cell cycle retardation and expansion of the precursor pool were transient. Cell cycle progression in the PTZ‐treated group returned to normal after one cell cycle. The numbers of precursors in the SVZ and new neurons in the olfactory bulb, which are descendants of SVZ precursors, were not significantly different from those in control mice more than 2 days after seizures. Because similar effects were observed following electroconvulsive seizures, these responses are likely to be general effects of brief seizures. These results suggest that neurogenesis in the SVZ is more tightly regulated and requires stronger stimuli to be modified than that in the SGZ.
Publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
Content Type
Journal Article
Link
ISSN
14609568
NCID
AA11622970
Journal Title
European journal of neuroscience
Volume
36
Issue
9
Start Page
3184
End Page
3193
Published Date
2012-11-30
Publisher-DOI
Text Version
Author
Rights
© 2012 The Authors. European Journal of Neuroscience © 2012 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
Citation
This is the pre-peer reviewed version of the following article: Mori, T., Wakabayashi, T., Hirahara, Y., Takamori, Y., Koike, T., Kurokawa, K. and Yamada, H. (2012), Differential responses of endogenous adult mouse neural precursors to excess neuronal excitation. European Journal of Neuroscience, 36: 3184-3193. doi:10.1111/j.1460-9568.2012.08244.x, which has been published in final form at https://doi.org/10.1111/j.1460-9568.2012.08244.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English