bbrc646(1)_269.pdf 504 KB
Koike, Taro Department of Anatomy and Cell Science, Kansai Medical University
Wakabayashi, Taketoshi Department of Anatomy and Cell Science, Kansai Medical University
Mori, Tetsuji Department of Anatomy and Cell Science, Kansai Medical University Researchers DB KAKEN
Hirahara, Yukie Department of Anatomy and Cell Science, Kansai Medical University
Yamada, Hisao Department of Anatomy and Cell Science, Kansai Medical University
Satellite glial cell
Sox2 is a transcriptional factor expressed in neural stem cells. It is known that Sox2 regulates cell differentiation, proliferation and survival of the neural stem cells. Our previous study showed that Sox2 is expressed in all satellite glial cells of the adult rat dorsal root ganglion. In this study, to examine the role of Sox2 in satellite glial cells, we establish a satellite glial cell-enriched culture system. Our culture method succeeded in harvesting satellite glial cells with the somata of neurons in the dorsal root ganglion. Using this culture system, Sox2 was downregulated by siRNA against Sox2. The knockdown of Sox2 downregulated ErbB2 and ErbB3 mRNA at 2 and 4 days after siRNA treatment. MAPK phosphorylation, downstream of ErbB, was also inhibited by Sox2 knockdown. Because ErbB2 and ErbB3 are receptors that support the survival of glial cells in the peripheral nervous system, apoptotic cells were also counted. TUNEL-positive cells increased at 5 days after siRNA treatment. These results suggest that Sox2 promotes satellite glial cell survival through the MAPK pathway via ErbB receptors.
Biochemical and biophysical research communications
Copyright © 2015 Elsevier Inc. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
Koike T., Wakabayashi T., Mori T., Hirahara Y., Yamada H. Sox2 promotes survival of satellite glial cells in vitro. Biochemical and Biophysical Research Communications(2015), 464 (1) , pp. 269-274.
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital