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Authors
Ogawa, Kenjirou Organization for Promotion of Tenure Track, University of Miyazaki
Ishii, Ayumi Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
Shindo, Aimi Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University
Hongo, Kunihiro Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University Researchers DB KAKEN
Mizobata, Tomohiro Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University Researchers DB KAKEN
Sogon, Tetsuya R&D Department,Wakasa Seikatsu Co. Ltd.
Kawata, Yasushi Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University Researchers DB KAKEN
Keywords
spearmint
rosmarinic acid
polyphenol
amyloid fibril
amyloid beta
alpha-synuclein
Tau
dementia
Abstract
Neurological dementias such as Alzheimer’s disease and Lewy body dementia are thought to be caused in part by the formation and deposition of characteristic insoluble fibrils of polypeptides such as amyloid beta (Aβ), Tau, and/or α-synuclein (αSyn). In this context, it is critical to suppress and remove such aggregates in order to prevent and/or delay the progression of dementia in these ailments. In this report, we investigated the effects of spearmint extract (SME) and rosmarinic acid (RA; the major component of SME) on the amyloid fibril formation reactions of αSyn, Aβ, and Tau proteins in vitro. SME or RA was added to soluble samples of each protein and the formation of fibrils was monitored by thioflavin T (ThioT) binding assays and transmission electron microscopy (TEM). We also evaluated whether preformed amyloid fibrils could be dissolved by the addition of RA. Our results reveal for the first time that SME and RA both suppress amyloid fibril formation, and that RA could disassemble preformed fibrils of αSyn, Aβ, and Tau into non-toxic species. Our results suggest that SME and RA may potentially suppress amyloid fibrils implicated in the progression of Alzheimer’s disease and Lewy body dementia in vivo, as well.
Publisher
MDPI
Content Type
Journal Article
Link
EISSN
20726643
Journal Title
NUTRIENTS
Volume
12
Issue
11
Published Date
2020-11
Publisher-DOI
Text Version
Publisher
Rights
(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Citation
Ogawa Kenjirou, Ishii Ayumi, Shindo Aimi, et al. Spearmint Extract Containing Rosmarinic Acid Suppresses Amyloid Fibril Formation of Proteins Associated with Dementia. NUTRIENTS. 2020. 12(11). doi:10.3390/nu12113480
Department
Faculty of Engineering/Graduate School of Engineering
Language
English
Web of Science Key ut
WOS:000593882900001