File
Authors
Keywords
apoptosis
bladder
estrogen
rat
Abstract
Effects of estradiol (E2) on the urinary bladder of old female rats were investigated by morphological, immunohistochemical and apoptosis-related gene analysis. Sixteen-month-old female Wistar rats were divided into the following 5 groups: normal controls; rats replaced with low-dose E2 for 2 and 4 weeks, respectively; and rats replaced with high-dose E2 for 2 and 4 weeks, respectively. After treatment, the bladders were removed, weighed and stained with hematoxylin and eosin, and immunostained by monoclonal antibodies against Bcl-2 and Bax proteins. Further, the expression of apoptosis-related genes bcl-2 and bax were investigated by PCR analysis, and evaluated by computerized quantification. Immunostained bladders showed no difference between control and E2-replaced rats in reactivity against Bcl-2 protein. In contrast, against Bax protein, control rats showed immunoreactivity, whereas E2-replaced rats showed little reactivity. Upon mRNA analysis, the expression of bax mRNA was suppressed in the E2-replaced rats as compared with the controls, though the expression of bcl-2 mRNA showed no difference among groups. These results suggest that E2 administration affects the expression of apoptosis-related genes and suppressees apoptotic action, and that these changes could consequently alter voiding behavior.
Publisher
Tottori University Faculty of Medicine
Content Type
Journal Article
ISSN・ISBN
1346-8049
NCID
AA00892883
Journal Title
Yonago Acta medica
Current Journal Title
Yonago Acta medica
Volume
45
Issue
4
Start Page
95
End Page
102
Published Date
2002-12
Text Version
Publisher
Rights
Yonago Acta medica 編集委員会
Citation
Yonago Acta medica. 2002, 45(3), 95-102
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English