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Authors
Matsuura, Kazunori Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University Researchers DB KAKEN
Nakamura, Tomohiro Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Watanabe, Kenta Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Noguchi, Takanori Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Minamihata, Kosuke Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Kamiya, Noriho Department of Applied Chemistry, Graduate School of Engineering, Kyushu University/Division of Biotechnology, Center for Future Chemistry, Kyushu University
Kimizuka, Nobuo Department of Applied Chemistry, Graduate School of Engineering, Kyushu University/Center for Molecular Systems (CMS), Kyushu University
Abstract
β-Annulus peptide bearing Cys at the N-terminal from tomato bushy stunt virus was synthesised using a standard Fmoc-protected solid-phase method, and the petide was modified with Ni-NTA at the N-terminal. The Ni-NTA-modified β-annulus peptide self-assembled into virus-like nanocapsules of approximately 40 nm in diameter. The critical aggregation concentration of these nanocapsules in 10 mM Tris-HCl buffer (pH 7.3) at 25°C was 0.053 μM, which is 470 times lower than that of unmodified β-annulus peptides. Moreover, size exclusion chromatography of the peptide assembly indicated encapsulation of His-tagged green fluorescent protein in the Ni-NTA-modified artificial viral capsid.
Publisher
Royal Society of Chemistry
Content Type
Journal Article
Link
ISSN
14770520
NCID
AA12084375
Journal Title
Organic & biomolecular chemistry
Current Journal Title
Organic & biomolecular chemistry
Volume
14
Issue
33
Start Page
7869
End Page
7874
Published Date
2016
Publisher-DOI
Text Version
Author
Rights
© The Royal Society of Chemistry 2016
Citation
This work was published by The Royal Society of Chemistry: K. Matsuura, T. Nakamura, K. Watanabe, T. Noguchi, K. Minamihata, N. Kamiyab and N. Kimizuka. Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation of His-tagged proteins. Organic & biomolecular chemistry. 2016, 14, 7869-7874
Department
Faculty of Engineering/Graduate School of Engineering
Language
English