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Authors
Saito, Kengo Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University
Koike, Taro Department of Anatomy and Cell Science, Kansai Medical University
Kawashima, Fumiaki Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University
Kurata, Hirofumi Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University / Division of Child Neurology, Department of Brain and Neurosciences, Faculty of Medicine, Tottori University Researchers DB KAKEN
Shibuya, Taku Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Sciences
Satoh, Takemasa Division of Neurobiology, School of Life Sciences, Faculty of Medicine, Tottori University Researchers DB KAKEN
Hata, Yoshio Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Sciences / Division of Neurobiology, School of Life Sciences, Faculty of Medicine, Tottori University Researchers DB KAKEN
Yamada, Hisao Department of Anatomy and Cell Science, Kansai Medical University
Mori, Tetsuji Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University Researchers DB KAKEN
Keywords
adult neurogenesis
heterogenity
neurogenic niche
NeuN
subventricular zone
Abstract
In the adult rodent subventricular zone (SVZ), there are neural stem cells (NSCs) and the specialized neurogenic niche is critical to maintain their stemness. To date, many cellular and noncellular factors that compose the neurogenic niche and markers to identify subpopulations of Type A cells have been confirmed. In particular, neurotransmitters regulate adult neurogenesis and mature neurons in the SVZ have been only partially analyzed. Moreover, Type A cells, descendants of NSCs, are highly heterogeneous and more molecular markers are still needed to identify them. In the present study, we systematically classified NeuN, commonly used as a marker of mature and immature post‐mitotic neurons, immunopositive (+) cells within the adult mouse SVZ. These SVZ‐NeuN+ cells (SVZ‐Ns) were mainly classified into two types. One was mature SVZ‐Ns (M‐SVZ‐Ns). Neurochemical properties of M‐SVZ‐Ns were similar to those of striatal neurons, but their birth date and morphology were different. M‐SVZ‐Ns were generated during embryonic and early postnatal stages with bipolar peaks and extended their processes along the wall of the lateral ventricle. The second type was small SVZ‐Ns (S‐SVZ‐Ns) with features of Type A cells. They expressed not only markers of Type A cells, but also proliferated and migrated from the SVZ to the olfactory bulb. Furthermore, S‐SVZ‐Ns could be classified into two types by their spatial locations and glutamic acid decarboxylase 67 expression. Our data indicate that M‐SVZ‐Ns are a new component of the neurogenic niche and S‐SVZ‐Ns are newly identified subpopulations of Type A cells.
Publisher
Wiley Periodicals
Content Type
Journal Article
Link
ISSN・ISBN
10969861
NCID
AA11628274
Journal Title
The Journal of comparative neurology
Volume
526
Issue
12
Start Page
1927
End Page
1942
RESEARCH ARTICLE
Published Date
2018-05-11
Publisher-DOI
Text Version
Author
Rights
© 2018 Wiley Periodicals, Inc.
Citation
This is the peer reviewed version of the following article: Saito, K, Koike, T, Kawashima, F, et al. Identification of NeuN immunopositive cells in the adult mouse subventricular zone. J Comp Neurol. 2018; 526: 1927– 1942. https://doi.org/10.1002/cne.24463, which has been published in final form at https://doi.org/10.1002/cne.24463. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English