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Authors
Fujiwara, Kazunori Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine Researchers DB KAKEN
Yazama, Hiroaki Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine Researchers DB KAKEN
Donishi, Ryohei Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine
Koyama, Satoshi Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine Researchers DB
Fukuhara, Takahiro Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine Researchers DB KAKEN
Kitatani, Kazuyuki Laboratory of Immunopharmacology, Faculty of Pharmaceutical Sciences, Setsunan University
Kataoka, Hideyuki Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine Researchers DB KAKEN
Takeuchi, Hiromi Division of Otolaryngology, Head and Neck Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine Researchers DB KAKEN
Abstract
Background: Anaplastic thyroid carcinoma (ATC) is an aggressive type of thyroid cancer, and its metastasis requires cell motility. Ceramide is involved in a variety of biological processes, including inflammation, cell signaling, cell motility, and induction of apoptosis, however has not previously been reported to inhibit the motility of ATC cells. We evaluated the effect of short chain C6-ceramide on motility of ATC cells.

Methods: Cell motility of 8305C thyroid carcinoma cell line treated with C6-ceramide was assessed using a transwell migration assay and a pseudopodia formation assay.

Results: Treatment with 10 μM C6-ceramide resulted in significantly fewer migratory cells than control treatment in a transwell migration assay (P < 0.002). In condition medium, 82.6% of C6-ceramide–treated cells formed lamellipodia. Importantly, treatment with 10
μM C6-ceramide drastically decreased the number of cells forming lamellipodia by 17.6% (P < 0.01).

Conclusion: Our results suggest that treatment with a low concentration of ceramide may prevent metastasis and recurrence of ATC by inhibiting cell motility. Further studies are necessary to investigate the mechanism of inhibition of cell motility by ceramide. Ceramide shows promise as a therapeutic treatment for ATC.
Publisher
Tottori University Medical Press
Content Type
Journal Article
Link
ISSN
05135710
NCID
AA00892882
Journal Title
Yonago Acta Medica
Current Journal Title
Yonago Acta Medica
Volume
63
Issue
2
Start Page
95
End Page
98
Published Date
2020-05-18
Publisher-DOI
Text Version
Publisher
Rights
注があるものを除き、この著作物は日本国著作権法により保護されています。 / This work is protected under Japanese Copyright Law unless otherwise noted.
Citation
K. Fujiwara, H. Yazama, R. Donishi, et al. C6-ceramide Inhibits the Motility of Anaplastic Thyroid Carcinoma Cells. Yonago Acta Medica. 2020, 63(2), 95-98. doi:10.33160/yam.2020.05.001
Department
Faculty of Medicine/Graduate School of Medical Sciences/University Hospital
Language
English