ID 8275
フルテキストファイル
著者
Morita, Maya Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University
Sawaki, Kazumasa Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University
Kinoshita, Daiki Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University
Sakurai, Chiye Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University 研究者総覧 KAKEN
Hori, Naohiro Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University 研究者総覧 KAKEN
Hatsuzawa, Kiyotaka Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University 研究者総覧 KAKEN
キーワード
Escherichia coli
macrophage
phagocytosis
phagosome maturation
tandem fluorescent protein
抄録
Phagosome formation and maturation are essential innate immune mechanisms to engulf and digest foreign particles. To analyze these processes quantitatively, we established a specific Escherichia coli probe expressing a tandem fluorescent protein, comprising glutathione S-transferase fused with monomeric Cherry (mCherry) and monomeric Venus (mVenus). We demonstrated that mVenus was more susceptible to bleaching in an acidic environment than mCherry, and that the mVenus:mCherry fluorescence intensity ratio can be used to monitor phagosomal pH changes during maturation. Using this probe, we revealed that synaptosomal-associated protein of 23 kDa, a plasma membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein, actively regulated phagocytosis of E. coli and subsequent phagosome maturation in macrophages. Our results indicated that this probe has the potential to be a powerful tool in understanding the molecular mechanisms of phagosome formation and maturation.
出版者
Oxford University Press
資料タイプ
学術雑誌論文
外部リンク
ISSN
0021924X
EISSN
17562651
掲載誌名
JOURNAL OF BIOCHEMISTRY
最新掲載誌名
JOURNAL OF BIOCHEMISTRY
162
5
開始ページ
309
終了ページ
316
発行日
2017-11
出版者DOI
著者版フラグ
著者版
著作権表記
This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of biochemistry following peer review. The version of record Morita Maya, Sawaki Kazumasa, Kinoshita Daiki, et al. Quantitative analysis of phagosome formation and maturation using an Escherichia coli probe expressing a tandem fluorescent protein. JOURNAL OF BIOCHEMISTRY. 2017. 162(5). 309-316. doi:10.1093/jb/mvx034 is available online at: https://academic.oup.com/jb/article/162/5/309/3858071
掲載情報
Morita Maya, Sawaki Kazumasa, Kinoshita Daiki, et al. Quantitative analysis of phagosome formation and maturation using an Escherichia coli probe expressing a tandem fluorescent protein. JOURNAL OF BIOCHEMISTRY. 2017. 162(5). 309-316. doi:10.1093/jb/mvx034
部局名
医学部・医学系研究科・医学部附属病院
言語
英語
Web of Science Key ut
WOS:000414360000001