フルテキストファイル
著者
Kazuyama Emi Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University
Saito Motoaki Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University KAKEN
Kinoshita Yukako Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University KAKEN
Satoh Itaru Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University
Dimitriadis Fotios Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University
Satoh Keisuke Division of Molecular Pharmacology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University KAKEN
キーワード
Diabetes
Goto-Kakizaki (GK) rat
Aorta
Nitric oxide synthase
Aging
抄録
As there are increasing evidences that human diabetes induces cardiovascular dysfunction, we investigated the type-2 diabetes-induced endothelial dysfunction in the early and late-stage Goto-Kakizaki (GK) rat aorta. We performed organ bath studies, and examined the changes in expression levels of muscarinic M(3) receptor, endothelial, inducible, and neuronal nitric oxide synthase (eNOS, iNOS, and nNOS, respectively) mRNAs in the rat aorta utilizing real-time polymerase chain reaction in 12-week-old and 70-week-old GK rats as well as in age-matched Wistar rats. In the 12-week-old GK rat aorta, a significant increase in norepinephrine-induced contraction and a significant decrease in acetylcholine-induced relaxation as well as significant increases in expression levels of muscarinic M(3) receptor and eNOS and a significant decrease in nNOS mRNAs were observed compared to age-matched controls. In the older GK rat aorta, significant decreases in acetylcholine- and nitroglycerine-induced relaxations as well as significant decreases in the expression levels of muscarinic M(3) receptor, eNOS, iNOS, and nNOS mRNAs were observed compared to those in the younger GK rats. In contrast, although significant decreases in acetylcholine and nitroglycerine-induced relaxations were observed, the expression levels of muscarinic M(3) receptor, eNOS, iNOS, and nNOS mRNAs in the older Wistar rats aorta were unchanged, increased, increased and decreased, respectively, compared to the younger Wistar rat aorta. These results indicate that endothelial dysfunction in the rat aorta progresses with age and development of diabetes condition, and that decreased relaxations in the late-stage rat aorta may be due to these alterations.
出版者
Springer
資料タイプ
学術雑誌論文
外部リンク
ISSN
0300-8177
書誌ID
AA00745800
掲載誌名
Molecular and Cellular Biochemistry
最新掲載誌名
Molecular and Cellular Biochemistry
332
1-2
開始ページ
95
終了ページ
102
発行日
2009-12
出版者DOI
著者版フラグ
著者版
著作権表記
The final publication is available at www.springerlink.com
掲載情報
Molecular and Cellular Biochemistry 2009, 332(1-2):95–102
部局名
医学部・医学系研究科・医学部附属病院
言語
英語