フルテキストファイル | |
著者 |
Kishimoto Takuji
Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine
KAKEN
Kaetsu Akihiko
Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine
KAKEN
Osaki Yoneatsu
Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine
研究者総覧
KAKEN
Okamoto Mikizoh
Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine
KAKEN
Nagai Mariya
Division of Environmental and Preventive Medicine, Tottori University Faculty of Medicine
Kurosawa Yoichi
Division of Health Administration and Promotion, Department of Social Medicine, Tottori University Faculty of Medicine
研究者総覧
KAKEN
Yoshida Soichi
Division of Reproductive-Perinatal Medicine and Gynecologic Oncology, Department of Surgery, School of Medicine, Tottori University Faculty of Medicine
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キーワード | gene expression
nitric oxide synthase
polymorphism
vascular endothelial cell
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抄録 | The relationship between cardiovascular disease and Glu298Asp polymorphism of nitric oxide synthase (eNOS) gene in vascular endothelial cells is controversial due to conflicting results. To demonstrate the effects of Glu298Asp polymorphism of the eNOS gene on eNOS gene expression by excluding the effects of various confounding factors, eNOS mRNA and protein expressions were measured using cultured human umbilical vein endothelial cells (HUVECs) according to genotypes of Glu298Asp polymorphism of the eNOS gene. eNOS mRNA expression was measured by real-time PCR/reverse trasnscription-PCR. eNOS protein expression was measured by an immunoassay method. HUVECs with Glu298Asp tended to show higher eNOS mRNA and protein expressions than those with Glu298Glu, but the difference was not significant. These results suggest that the genotypic difference of Glu298Glu and Glu298Asp has no effect on eNOS mRNA and protein expressions.
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出版者 | Tottori University Faculty of Medicine
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資料タイプ |
学術雑誌論文
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ISSN | 1346-8049
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書誌ID | AA00892882
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掲載誌名 | Yonago Acta medica
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最新掲載誌名 |
Yonago Acta medica
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巻 | 49
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号 | 1
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開始ページ | 59
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終了ページ | 62
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発行日 | 2006-03
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著者版フラグ |
出版社版
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著作権表記 | Yonago Acta medica 編集委員会
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掲載情報 | Yonago Acta medica. 2006, 49(1), 59-62
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部局名 |
医学部・医学系研究科・医学部附属病院
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言語 |
英語
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