フルテキストファイル
著者
Suzuki Yoshimasa Second Department of Surgery, Faculty of Medicine, Tottori University 研究者総覧 KAKEN
Tanaka Yoshiyuki Second Department of Surgery, Faculty of Medicine, Tottori University
Nakamura Hiroshige Second Department of Surgery, Faculty of Medicine, Tottori University 研究者総覧 KAKEN
Ohgi Shigetsugu Second Department of Surgery, Faculty of Medicine, Tottori University KAKEN
キーワード
human lung cancer
interferon-gamma
major histocompatibility complex
tyrosine phosphorylation
抄録
Expression of major histocompatibility complex (MHC) antigens on cancer cells is essential for cell-mediated immune function. However, these molecules are reduced on cancer cells enabling them to escape from host immune surveillance. It is well known that interferon-γ (IFN-γ) upregulates the expression of MHC molecules and restores the immunogenicity of cancer cells. Nevertheless, the mechanism by which IFN-γ modulates MHC expression on cancer cells is not clear. Therefore, in this report, we examined the role of tyrosine protein kinases in IFN-γ-induced MHC expression in a human lung adenocarcinoma cell line, HLC-1. We found that a tyrosine protein kinase inhibitor, herbimycin A, inhibited both IFN-γ-inducible MHC class I and class II expression, as assessed by flow cytometry. Additionally, assessment of tyrosine phosphorylation of cellular substrates by confocal laser microscopy using an anti-phosphotyrosine monoclonal antibody (mAb) revealed that IFN-γ induced protein tyrosine phosphorylation within 5 min of treatment. Herbimycin A inhibited this IFN-γ-induced tyrosine phosphorylation. Thus, tyrosine phosphorylation plays an important role in IFN-γ-induced MHC class I and class II expression on HLC-1 cells.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
41
1
開始ページ
1
終了ページ
6
発行日
1998-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 1998, 41(1), 1-6
部局名
医学部・医学系研究科・医学部附属病院
言語
英語