フルテキストファイル
著者
Yanagi Koji Department of Urology, Tottori University, Faculty of Medicine
キーワード
bladder outlet obstruction
nitric oxide
nitric oxide synthase activity
L-arginine
抄録
We investigated the changes in bladder function resulting from bladder outlet obstruction (BOO), with particular emphasis on the role of nitric oide (NO) in bladder response. Twelve-week-old female Wistar rats (n = 36) were surgically treated in various ways by dividing them into 6 groups each with 6 animals: 2 sham-operated groups, 2 obstructed groups for 1 week or 6 weeks, and 2 groups obstructed and intraperitoneally injected with 150-mg/kg L-arginine once daily for 1 week or 6 weeks. We conducted bladder strip stimulation studies using carbachol and KCl. Then the bladder was used for measurement of NO synthase (NOS) activity and was also studied histologically using immunohistochemical staining. Bladders in the obstructed groups were significantly heavier than in the sham-operated groups. The mean weight of bladders in the groups obstructed and treated with L-arginine was almost the same as that in the sham-operated groups. Tissue bath studies demonstrated decreased contractility in response to cholinergic stimulation at obstruction, but contractility at obstruction was improved by L-arginine for 1 week or 6 weeks. NOS activity in bladder tissue was lower in the obstructed groups than in the sham groups, and higher in the L-arginine-combined groups than in the obstructed groups. These results indicate that bladder dysfunction may be improved by treatment with L-arginine. This study suggests that increased NO by treatment with L-arginine plays a role in improving bladder dysfunction caused by BOO.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
45
1
開始ページ
19
終了ページ
26
発行日
2002-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2002, 45(1), 19-26
部局名
医学部・医学系研究科・医学部附属病院
言語
英語