フルテキストファイル
著者
尾崎 弘一 Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University / Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University 研究者総覧 KAKEN
Yonehara, K. Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University
村瀬 敏之 Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University / Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University 研究者総覧 KAKEN
キーワード
avian pathogenic Escherichia coli
chicken
colibacillosis
embryo lethality assay
infection model
virulence
抄録
To evaluate the virulence of avian pathogenic Escherichia coli (APEC) isolates obtained from colibacillosis cases associated with pericarditis, perihepatitis, and salpingitis, the embryo lethality assay and experimental infection model in chicks were used in this study. According to the established criteria based on mortality in the embryo lethality assay for evaluating the virulence of E. coli isolates, 23 of the 26 APEC isolates associated with pericarditis and perihepatitis and 8 of the 20 isolates associated with salpingitis were found to be virulent. Isolate D137, which had been obtained from a case with pericarditis and perihepatitis and had an embryo mortality of 92%, and isolate D445, which had been obtained from a case with pericarditis and perihepatitis and had an embryo mortality of 17%, were used for the experimental infection. Four of the five 11-day-old chickens inoculated through the air sac with isolate D137 died 1 day postinoculation, and the challenge strain was recovered from the air sac, pericardial sac, or liver; however, colibacillosis lesions were found in only one of the five birds postmortem. All five chicks inoculated with isolate D445 survived for 7 days postinoculation and exhibited airsacculitis or pericarditis lesions at 7 days postinoculation; the challenge strain was not recovered from the lesions postmortem. The results obtained in this study suggest that the different APEC isolates tested cause illness in chickens through distinct pathogenesis.
出版者
BioOne
資料タイプ
学術雑誌論文
外部リンク
ISSN
00052086
EISSN
19384351
掲載誌名
AVIAN DISEASES
62
2
開始ページ
233
終了ページ
236
発行日
2018-06
出版者DOI
著者版フラグ
その他
著作権表記
(C)2018 the American Association of Avian Pathologists
掲載情報
Ozaki H., Yonehara K., Murase T., et al. Virulence of Escherichia coli Isolates Obtained from Layer Chickens with Colibacillosis Associated with Pericarditis, Perihepatitis, and Salpingitis in Experimentally Infected Chicks and Embryonated Eggs. AVIAN DISEASES. 2018. 62(2). 233-236. doi:10.1637/11685-060717-ResNote.1
部局名
農学部・農学研究科
言語
英語
Web of Science Key ut
WOS:000436414700014