フルテキストファイル
著者
Mori, Tetsuji Department of Anatomy and Cell Science, Kansai Medical University 研究者総覧 KAKEN
Wakabayashi, Taketoshi Department of Anatomy and Cell Science, Kansai Medical University
Ogawa, Haruyuki Department of Anatomy and Cell Science, Kansai Medical University
Hirahara, Yukie Department of Anatomy and Cell Science, Kansai Medical University
Koike, Taro Department of Anatomy and Cell Science, Kansai Medical University
Yamada, Hisao Department of Anatomy and Cell Science, Kansai Medical University
抄録
Status epilepticus (SE) induces pathological and morphological changes in the brain. Recently, it has become clear that excessive neuronal excitation, stress and drug abuse induce chromatin remodeling in neurons, thereby altering gene expression. Chromatin remodeling is a key mechanism of epigenetic gene regulation. Histone H3 phosphorylation is frequently used as a marker of chromatin remodeling and is closely related to the upregulation of mRNA transcription. In the present study, we analyzed H3 phosphorylation levels in vivo using immunohistochemistry in the brains of mice with pilocarpine-induced SE. A substantial increase in H3 phosphorylation was detected in neurons in specific brain structures. Increased H3 phosphorylation was dependent on neuronal excitation. In particular, a robust upregulation of H3 phosphorylation was detected in the caudate putamen, and there was a gradient of phosphorylated H3+ (PH3+) neurons along the medio-lateral axis. After unilateral ablation of dopaminergic neurons in the substantia nigra by injection of 6-hydroxydopamine, the distribution of PH3+ neurons changed in the caudate putamen. Moreover, our histological analysis suggested that, in addition to the well-known MSK1 (mitogen and stress-activated kinase)/H3 phosphorylation/c-fos pathway, other signaling pathways were also activated. Together, our findings suggest that a number of genes involved in the pathology of epileptogenesis are upregulated in PH3+ brain regions, and that H3 phosphorylation is a suitable indicator of strong neuronal excitation.
出版者
PLOS
資料タイプ
学術雑誌論文
外部リンク
ISSN
19326203
掲載誌名
PLOS ONE
8
10
開始ページ
e77710
発行日
2013-10-16
出版者DOI
著者版フラグ
出版社版
著作権表記
© 2013 Mori et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
掲載情報
Mori T, Wakabayashi T, Ogawa H, Hirahara Y, Koike T, Yamada H (2013) Increased Histone H3 Phosphorylation in Neurons in Specific Brain Structures after Induction of Status Epilepticus in Mice. PLoS ONE 8(10): e77710. https://doi.org/10.1371/journal.pone.0077710
部局名
医学部・医学系研究科・医学部附属病院
言語
英語