フルテキストファイル
著者
Mori, Tetsuji Department of Anatomy & Cell Science, Kansai Medical University 研究者総覧 KAKEN
Wakabayashi, Taketoshi Department of Anatomy & Cell Science, Kansai Medical University
Takamori, Yasuharu Department of Anatomy & Cell Science, Kansai Medical University
Kitaya, Kotaro Department of Anatomy & Cell Science, Kansai Medical University
Yamada, Hisao Department of Anatomy & Cell Science, Kansai Medical University
キーワード
NG2
adult brain
precursor
quantification
抄録
In intact adult mammalian brains, there are two neurogenic regions: the subependymal zone and the subgranular layer of the hippocampus. Even outside these regions, small numbers of proliferating precursors do exist. Many studies suggest that the majority of these are oligodendrocyte precursors that express NG2, a chondroitin sulfate proteoglycan, and most of the residual proliferating cells seem to be endothelial cells. However, it is still unclear whether NG2-immunonegative proliferating precursors are present, because previous studies have neglected their possible existence. In this study, we systematically analyzed the phenotypes of the proliferating cells in the intact adult rat cortical gray matter. We improved our techniques and carefully characterized the proliferating cells, because there were several problems with identifying and quantifying the proliferating cells: the detection of NG2-expressing cells was dependent on the fixation condition; there were residual proliferating leukocytes in the blood vessels; and two anti-NG2 antibodies gave rise to different staining patterns. Moreover, we used two methods, BrdU and Ki67 immunostaining, to quantify the proliferating cells. Our results strongly suggest that in the intact adult cerebral cortical gray matter, there were only two types of proliferating cells: the majority were NG2-expressing cells, including pericytes, and the rest were endothelial cells.
出版者
Japan Society of Histochemistry and Cytochemistry
資料タイプ
学術雑誌論文
外部リンク
ISSN
13475800
書誌ID
AA1202220X
掲載誌名
Acta histochemica et cytochemica
42
1
開始ページ
1
終了ページ
8
REGULAR ARTICLE
発行日
2009
出版者DOI
著者版フラグ
出版社版
著作権表記
© 2009 By the Japan Society of Histochemistry and Cytochemistry. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
掲載情報
Tetsuji Mori, Taketoshi Wakabayashi, Yasuharu Takamori, Kotaro Kitaya, Hisao Yamada. Phenotype Analysis and Quantification of Proliferating Cells in the Cortical Gray Matter of the Adult Rat. ACTA HISTOCHEMICA ET CYTOCHEMICA, 2009, Volume 42, Issue 1, Pages 1-8
部局名
医学部・医学系研究科・医学部附属病院
言語
英語