フルテキストファイル
著者
Matsuura Haruyo Division of Medical Zoology, Department of Microbiology and Immunology, School of Medicine, Tottori University Faculty of Medicine KAKEN
Tomioku Asumi Division of Medical Zoology, Department of Microbiology and Immunology, School of Medicine, Tottori University Faculty of Medicine
Kono Satoko Division of Medical Zoology, Department of Microbiology and Immunology, School of Medicine, Tottori University Faculty of Medicine
Tademoto Sayuri Technical Support Section, Tottori University Faculty of Medicine
Fukumoto Soji Division of Medical Zoology, Department of Microbiology and Immunology, School of Medicine, Tottori University Faculty of Medicine 研究者総覧 KAKEN
キーワード
macrophage
chemokine
excretory/secretory products
lipopolysaccharide
Spirometra erinaceieuropaei
抄録
Previous studies have shown that excretory/secretory (ES) products from plerocercoids of Spirometra erinaceieuropaei have immunosuppressive activities. We report here that a 130 kDa glycoprotein (ES130) purified from ES products as a suppressive factor of nitric oxide synthesis in LPS-stimulated RAW 264.7 cells inhibited the gene expression of 3 chemokines, regulated on activation normal T cell expressed and secreted (CCL5/RANTES), macrophage inflammatory protein 2 (CXCL2/MIP-2), interferon-inducible protein 10 kDa (CXCL10/IP-10) in RAW 264.7 cells and mouse peritoneal macrophages stimulated with LPS for 3 h. These chemokines are important factors for recruitment of inflammatory leukocytes. RANTES acts on monocytes, basophils, lymphocytes, natural killer cells and eosinophils. MIP-2 is a potent chemoattactant for neutrophils, while IP-10 binds to Th1 cells. Nearly 80% of MIP-2 gene expression and 50% of IP-10 gene ex-pression in peritoneal macrophages stimulated with LPS for 8 h was suppressed as well as these chemokine production by the preincubation with 100 ng/mL of ES130 or 5000 ng/mL crude ES products for 24 h. On the other hand the mRNA expression of RANTES in macrophages stimulated with LPS for 8 h or 24 h was not inhibited by ES130 or crude ES products, while the RANTES chemokine levels in the incubation medium were significantly suppressed. These results suggest that ES130 may attenuate inflammation around the plerocercoids by inhibiting these chemokine production.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN・ISBN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
52
1
開始ページ
37
終了ページ
46
発行日
2009-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2009, 52(1), 37-46
部局名
医学部・医学系研究科・医学部附属病院
言語
英語