フルテキストファイル | |
著者 |
Nakamoto Sachiko
Department of Medical Technology, Tottori University, Institute of Medical Care Technology
KAKEN
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キーワード | Candida albicans
corn meal broth
germ tube formation
non-slip slide glass incubation method
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抄録 | Candida albicans IFO 1385 yeast cells dominantly develop germ tubes in a dilute corn meal broth at 37°C. For germ tube (GT) development the broth concentration is optimal at 75% of the original, and the inoculum is optimal at 105 cells/mL. Incubation procedures were compared and the non-slip slide glass incubation (NSSI) method was found to be more available than the tube incubation method or slid-covered slide glass incubation method. The GT formation was completed in 1 to 2 h in the NSSI method and clear-cut GTs were observed under optical microscopy. GTs were formed in all 111 test-strains of C. albicans but not at all in other species of Candida. Therefore, under optimal conditions, the rate of identifying C. albicans is 100%, demonstrating that GT detection with the NSSI method is a rapid and reliable identification method. The humid NSSI method is suited for continuous observation of a GT. The initially formed GT elongated to resemble a true hypha, attaching no chlamydoconidium. The hypha subsequently dissociated yeast-like cells.
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出版者 | Tottori University Faculty of Medicine
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資料タイプ |
学術雑誌論文
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ISSN | 1346-8049
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書誌ID | AA00892882
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掲載誌名 | Yonago Acta medica
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最新掲載誌名 |
Yonago Acta medica
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巻 | 41
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号 | 2
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開始ページ | 65
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終了ページ | 72
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発行日 | 1998-07
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著者版フラグ |
出版社版
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著作権表記 | Yonago Acta medica 編集委員会
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掲載情報 | Yonago Acta medica. 1998, 41(2), 65-72
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部局名 |
医学部・医学系研究科・医学部附属病院
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言語 |
英語
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