フルテキストファイル
著者
Buffo, Annalisa Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
Rite, Inmaculada Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health / Institute of Physiology, University of Munich
Tripathi, Pratibha Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
Lepier, Alexandra Institute of Physiology, University of Munich
Colak, Dilek Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
Horn, Ana-Paula Department of Biochemistry, Federal University of Rio Grande do Sul
Mori, Tetsuji Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health 研究者総覧 KAKEN
Götz, Magdalena Institute for Stem Cell Research, GSF-National Research Institute for Environment and Health
キーワード
astrocytes
cell fate
cerebral cortex
stem cells
抄録
Reactive gliosis is the universal reaction to brain injury, but the precise origin and subsequent fate of the glial cells reacting to injury are unknown. Astrocytes react to injury by hypertrophy and up-regulation of the glial-fibrillary acidic protein (GFAP). Whereas mature astrocytes do not normally divide, a subpopulation of the reactive GFAP+ cells does so, prompting the question of whether the proliferating GFAP+ cells arise from endogenous glial progenitors or from mature astrocytes that start to proliferate in response to brain injury. Here we show by genetic fate mapping and cell type-specific viral targeting that quiescent astrocytes start to proliferate after stab wound injury and contribute to the reactive gliosis and proliferating GFAP+ cells. These proliferating astrocytes remain within their lineage in vivo, while a more favorable environment in vitro revealed their multipotency and capacity for self-renewal. Conversely, progenitors present in the adult mouse cerebral cortex labeled by NG2 or the receptor for the platelet-derived growth factor (PDGFRα) did not form neurospheres after (or before) brain injury. Taken together, the first fate-mapping analysis of astrocytes in the adult mouse cerebral cortex shows that some astrocytes acquire stem cell properties after injury and hence may provide a promising cell type to initiate repair after brain injury.
出版者
National Academy of Sciences
資料タイプ
学術雑誌論文
外部リンク
ISSN
10916490
書誌ID
AA11726874
掲載誌名
PNAS : proceedings of the National Academy of Sciences of the United States of America
最新掲載誌名
PNAS : proceedings of the National Academy of Sciences of the United States of America
105
9
開始ページ
3581
終了ページ
3586
RESEARCH ARTICLE
発行日
2008-03-04
出版者DOI
著者版フラグ
著者版
著作権表記
© 2008 by The National Academy of Sciences of the USA
掲載情報
Annalisa Buffo, Inmaculada Rite, Pratibha Tripathi, Alexandra Lepier, Dilek Colak, Ana-Paula Horn, Tetsuji Mori, Magdalena Götz. Origin and progeny of reactive gliosis: A source of multipotent cells in the injured brain. Proceedings of the National Academy of Sciences Mar 2008, 105 (9) 3581-3586; DOI: 10.1073/pnas.0709002105
部局名
医学部・医学系研究科・医学部附属病院
言語
英語