フルテキストファイル
著者
Kashiwagi Ryota Second Department of Anatomy, Faculty of Medicine, Tottori University
Kaidoh Toshiyuki Second Department of Anatomy, Faculty of Medicine, Tottori University 研究者総覧 KAKEN
Inoue Takao Second Department of Anatomy, Faculty of Medicine, Tottori University KAKEN
キーワード
dendritic cell
griseofulvin
immunohistochemistry
Kupffer cell
protoporphyria
抄録
To determine whether protoporphyric liver injury affects hepatic dendritic cells (DC) and Kupffer cells, we examined liver tissues of griseofulvin-induced protoporphyric mice using histological and immunohistochemical methods. After 1 week of griseofulvin feeding, the protoporphyric liver showed prominent hepatomegaly and a gradual increase in histopathological changes such as hepatocellular hypertrophy, focal necrosis and brown pigment deposits. After 4 weeks of treatment, marked ductular reaction was observed in the liver tissue. Immunohistochemical analyses indicated that the density of NLDC-145-positive hepatic DC gradually decreased during griseofulvin feeding. However, the index of the number of DC in the whole liver appeared to fall sharply after 6 weeks. In contrast, the density of F4/80-positive Kupffer cells gradually increased during griseofulvin feeding. In the spleen and lung, no significant differences were noted in the distribution of NLDC-145-positive DC between experimental and control mice. These results suggested that griseofulvin-induced protoporphyria leads to a specific decrease in the density of hepatic DC due to hepatomegaly until 4 weeks of treatment and is substantial after 6 weeks. This substantial decrease of hepatic DC might have been induced by some alterations in protoporphyric liver injury including ductular reaction at a later stage in this experiment. Since hepatic DC were reduced in number, they seemed to have no significant relation to the progression of griseofulvin-induced protoporphyric liver injury. However, the decrease of hepatic DC might affect the cellular immune response in protoporphyria.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
44
1
開始ページ
7
終了ページ
16
発行日
2001-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2001, 44(1), 7-16
部局名
医学部・医学系研究科・医学部附属病院
言語
英語