フルテキストファイル
著者
Kometani Yasushi Third Department of Internal Medicine, Faculty of Medicine, Tottori University
Tomita Katsuyuki Third Department of Internal Medicine, Faculty of Medicine, Tottori University
Hitsuda Yutaka Third Department of Internal Medicine, Faculty of Medicine, Tottori University
キーワード
apoptosis
A549
laser scanning cytometry
terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method
抄録
Though cellular localization at the site of an inflammatory challenge is the critical first step in a neutrophil response, little has been known about the effects of alveolar epithelial cells on the dynamics of neutrophils. To investigate these effects, we used human purified neutrophils incubated with monolayers of human alveolar epithelial cells (A549), quiescent or preactivated with recombinant human tumor necrosis factor (TNF)-α for 24 h. Laser scanning cytometry (LSC) was employed to detect nuclear morphological changes and quantitate DNA strand breaks in neutrophils. The experiments revealed that A549 cells inhibited neutrophil apoptosis, and that this inhibitory effect was enhanced when the A549 cells were preactivated with TNF-α for 24 h. These results suggest that alveolar epithelial cells may be potentially able to contribute to promote inflammatory mechanisms by delaying neutrophil apoptosis.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
42
1
開始ページ
21
終了ページ
29
発行日
1999-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 1999, 42(1), 21-29
部局名
医学部・医学系研究科・医学部附属病院
言語
英語