フルテキストファイル
著者
Narita Akiko Second Department of Physiology and Department of Ophthalmology, Faculty of Medicine, Tottori University
Yawata Kenji Second Department of Physiology and Department of Ophthalmology, Faculty of Medicine, Tottori University
Nagata Masao Department of Ophthalmology, Faculty of Medicine, Tottori University
Aoyama Yasuaki Second Department of Physiology, Faculty of Medicine, Tottori University
Kawai Yasuaki Second Department of Physiology, Faculty of Medicine, Tottori University KAKEN
キーワード
ATP-induced calcium mobilization
extracellular acidification
intracellular pH
rabbit lens epithelial cells
抄録
Effects of extracellular acidification on intracellular pH (pHi) and ATP-induced calcium mobilization were investigated in rabbit lens epithelial cells. Primary-cultured lens epithelial cells of Japanese white rabbits were used. Intracellular calcium ([Ca2+]i) and pHi were measured by using fluorescent dyes, fura-2 acetoxymethylester (fura-2 AM) and 2',7'-bis (carboxyethyl)-5,6-carboxyfluorescein acetoxymethylester (BCECF AM), respectively. The addition of 10 ?mol/L ATP produced an initial peak followed by a sustained increase in [Ca2+]i in a standard artificial aqueous humor at extracellular pH (pH0) 7.40. The initial peak was abolished by pretreatment with 1 ?mol/L thapsigargin, whereas the sustained increase was attenuated in a Ca2+-free solution or by pretreatment with 100 ?mol/L verapamil. Acidification of the pH0 from 7.40 to 6.80 decreased the pHi from 7.21 to 7.03, and enhanced both the initial peak and sustained increase in [Ca2+]i. These results suggest that acidification of pH0 significantly affects the pHi and modifies the ATP-induced [Ca2+]i transient in rabbit lens epithelial cells.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
42
1
開始ページ
51
終了ページ
59
発行日
1999-03
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 1999, 42(1), 51-59
部局名
医学部・医学系研究科・医学部附属病院
言語
英語