フルテキストファイル
著者
Noguchi Makoto Industrial Research Institute of Tottori Prefecture
Takahashi Yusuke Industrial Research Institute of Tottori Prefecture, Division of Molecular Biology, Department of Molecular and Cellular Biology, Tottori University Faculty of Medicine
Taniguchi Makoto Division of Molecular Biology, Department of Molecular and Cellular Biology, Tottori University Faculty of Medicine
Isa Shun-ichi Division of Molecular Biology, Department of Molecular and Cellular Biology, Tottori University Faculty of Medicine
Arifuku Ichiro Industrial Research Institute of Tottori Prefecture
Yamashita Akimichi Industrial Research Institute of Tottori Prefecture
Sato Kenzo Division of Molecular Biology, Department of Molecular and Cellular Biology, Tottori University Faculty of Medicine KAKEN
キーワード
dermatan sulfate
fish skin
human hepatoma cell 3γ
liver function
reporter assay
抄録
The skin of the halibut (Reinhararditus hippoglossoides) has long been discarded as a waste product by the food industry in large quantities; hence, we examined the biological activity in the skin for utilization of the waste. In the present study, we determined induction of hepatocyte nuclear factor 3γ (HNF3γ), which is involved in liver function, using human hepatoma cell line HepG2 and extracellular matrix components extracted from the fish skin. Induction of the HNF3γ was detected by reporter plasmid constructed with the promoter region of the HNF3γ gene and luciferase or green fluorescent protein genes. The alkali extract containing proteoglycan showed HNF3γ induction, but the acid-extract enriched in collagen did not. Consequently, dermatan sulfate showed the strongest activity in HNF3γ induction. These observations suggested that fish skin has some significant biological activity, and indicates its possible function in the food and drug industry.
出版者
Tottori University Faculty of Medicine
資料タイプ
学術雑誌論文
ISSN・ISBN
1346-8049
書誌ID
AA00892882
掲載誌名
Yonago Acta medica
最新掲載誌名
Yonago Acta medica
46
4
開始ページ
103
終了ページ
108
発行日
2003-12
著者版フラグ
出版社版
著作権表記
Yonago Acta medica 編集委員会
掲載情報
Yonago Acta medica. 2003, 46(4), 103-108
部局名
医学部・医学系研究科・医学部附属病院
言語
英語